Monday Lecture Series
(open to the Rockefeller community)
Monday, October 17, 2022
Seth A. Darst, Ph.D.
Jack Fishman Professor,
Head of the Laboratory of Molecular Biophysics,
The Rockefeller University
Promoter Melting by Bacterial RNA Polymerase: Cryo-EM at Equilibrium and Non-equilibrium
Recommended Readings:
Empirical Articles
Malone, B., Campbell, E. A., & Darst, S. A. (2021). CoV-er all the bases: Structural perspectives of SARS-CoV-2 RNA synthesis. The Enzymes, 49, 1–37.
Chen, J., Wang, Q., Malone, B., Llewellyn, E., Pechersky, Y., Maruthi, K., Eng, E. T., Perry, J. K., Campbell, E. A., Shaw, D. E., & Darst, S. A. (2022). Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex. Nature structural & molecular biology, 29(3), 250–260.
Saecker, R. M., Chen, J., Chiu, C. E., Malone, B., Sotiris, J., Ebrahim, M., Yen, L. Y., Eng, E. T., & Darst, S. A. (2021). Structural origins of Escherichia coli RNA polymerase open promoter complex stability. Proceedings of the National Academy of Sciences of the United States of America, 118(40), e2112877118.
Malone, B., Chen, J., Wang, Q., Llewellyn, E., Choi, Y. J., Olinares, P., Cao, X., Hernandez, C., Eng, E. T., Chait, B. T., Shaw, D. E., Landick, R., Darst, S. A., & Campbell, E. A. (2021). Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex. Proceedings of the National Academy of Sciences of the United States of America, 118(19), e2102516118.
Kang, J. Y., Llewellyn, E., Chen, J., Olinares, P., Brewer, J., Chait, B. T., Campbell, E. A., & Darst, S. A. (2021). Structural basis for transcription complex disruption by the Mfd translocase. eLife, 10, e62117.
