Recommended Readings: Richard Lifton, M.D., Ph.D., June 10

Friday Lecture Series
Friday, June 10, 2016
3:45 p.m., Caspary Auditorium

Richard Lifton, M.D., Ph.D.
President-elect, The Rockefeller University
Sterling Professor of Genetics and Professor of Medicine (Nephrology),
Chair, Department of Genetics, Yale University
Investigator, Howard Hughes Medical Institute

From Genes and Genomes to Biology and Health

Recommended Readings:

Choi, M., Scholl, U. I., Ji, W., Liu, T., Tikhonova, I. R., Zumbo, P., … & Lifton, R. O. (2009). Genetic diagnosis by whole exome capture and massively parallel DNA sequencing. Proceedings of the National Academy of Sciences, 106(45), 19096-19101. doi: 10.1073/pnas.0910672106.

Ji, W., Foo, J. N., O’Roak, B. J., Zhao, H., Larson, M. G., Simon, D. B., … & Lifton, R. P. (2008). Rare independent mutations in renal salt handling genes contribute to blood pressure variation. Nature Genetics, 40(5), 592-599. doi: 10.1038/ng.118.

Lifton, R. P., Gharavi, A. G., & Geller, D. S. (2001). Molecular mechanisms of human hypertension. Cell, 104(4), 545-556. doi:10.1016/S0092-8674(01)00241-0

Shimkets, R. A., Warnock, D. G., Bositis, C. M., Nelson-Williams, C., Hansson, J. H., Schambelan, M., … & Lifton, R. O. (1994). Liddle’s syndrome: heritable human hypertension caused by mutations in the β subunit of the epithelial sodium channel. Cell, 79(3), 407-414. doi:10.1016/0092-8674(94)90250-X

Recommended Readings: Mary Hynes, Ph.D., May 23

Monday Lecture Series
Monday, May 23, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Mary Hynes, Ph.D.
Research Associate Professor,
Laboratory of Neural Specification and Development,
The Rockefeller University

Widespread Differential Expression of 3’ UTR and Cognate Coding Region Sequences in Neurons and Other Tissues

Recommended Readings:

Heiman, M., Kulicke, R., Fenster, R. J., Greengard, P., & Heintz, N. (2014). Cell type–specific mRNA purification by translating ribosome affinity purification (TRAP). Nature Protocols, 9(6), 1282-1291. doi: 10.1038/nprot.2014.085.

Kocabas, A., Duarte, T., Kumar, S., & Hynes, M. A. (2015). Widespread differential expression of coding region and 3′ UTR sequences in neurons and other tissues. Neuron, 88(6), 1149-1156. doi: 10.1016/j.neuron.2015.10.048.

Recommended Readings: Sean Brady, Ph.D., May 16

Monday Lecture Series
Monday, May 16, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Sean Brady, Ph.D.
Tri-Institutional Associate Professor,
Evnin Associate Professor and Head,
Laboratory of Genetically Encoded Small Molecules,
The Rockefeller University
Early Career Scientist, Howard Hughes Medical Institute

Bioactive Metabolites from the Human Microbiome: Natural Cures or Snake Oil

Recommended Readings:

Cohen, L. J., Kang, H. S., Chu, J., Huang, Y. H., Gordon, E. A., Reddy, B. V. B., … & Brady, S. F. (2015). Functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist. Proceedings of the National Academy of Sciences, 112(35), E4825-E4834. doi: 10.1073/pnas.1508737112.

Charlop-Powers, Z., Milshteyn, A., & Brady, S. F. (2014). Metagenomic small molecule discovery methods. Current Opinion in Microbiology, 19, 70-75. doi: 10.1016/j.mib.2014.05.021.

Recommended Readings: Carolyn Bertozzi, Ph.D., June 3

Friday Lecture Series
Friday, June 3, 2016
3:45 p.m., Caspary Auditorium

Carolyn Bertozzi, Ph.D.,
Anne T. and Robert M. Bass Professor of Chemistry,
Professor of Chemical & Systems Biology and Radiology (By Courtesy),
Stanford University
Investigator, Howard Hughes Medical Institute

Precision Glycocalyx Editing for Cancer Immune Therapy

Recommended Readings:

Agarwal, P., Beahm, B. J., Shieh, P., & Bertozzi, C. R. (2015). Systemic fluorescence imaging of zebrafish glycans with bioorthogonal chemistry. Angewandte Chemie, 127(39), 11666-11672. doi: 10.1002/anie.201504249.

Agarwal, P., & Bertozzi, C. R. (2015). Site-specific antibody–drug conjugates: The nexus of bioorthogonal chemistry, protein engineering, and drug development. Bioconjugate Chemistry, 26(2), 176-192. doi: 10.1021/bc5004982.

Hudak, J. E., Canham, S. M., & Bertozzi, C. R. (2014). Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion. Nature Chemical Biology, 10(1), 69-75. doi: 10.1038/nchembio.1388.

Woods, E. C., Yee, N. A., Shen, J., & Bertozzi, C. R. (2015). Glycocalyx engineering with a recycling glycopolymer that increases cell survival in vivo. Angewandte Chemie, 127(52), 16008-16014. doi: 10.1002/anie.201508783.

Recommended Readings: Angelika Amon, Ph.D., May 27

Friday Lecture Series
Friday, May 27, 2016
3:45 p.m., Caspary Auditorium

Angelika Amon, Ph.D.
Kathleen and Curtis Marble Professor of Cancer Research,
Koch Institute for Integrative Cancer Research,
Massachusetts Institute of Technology
Investigator, Howard Hughes Medical Institute

The Causes and Consequences of Aneuploidy

Recommended Readings:

Sheltzer, J. M., Blank, H. M., Pfau, S. J., Tange, Y., George, B. M., Humpton, T. J., … & Amon, A. (2011). Aneuploidy drives genomic instability in yeast. Science, 333(6045), 1026-1030. doi: 10.1126/science.1206412.

Sheltzer, J. M., Torres, E. M., Dunham, M. J., & Amon, A. (2012). Transcriptional consequences of aneuploidy. Proceedings of the National Academy of Sciences, 109(31), 12644-12649. doi: 10.1073/pnas.1209227109.

Tang, Y. C., & Amon, A. (2013). Gene copy-number alterations: a cost-benefit analysis. Cell, 152(3), 394-405. doi: 10.1016/j.cell.2012.11.043.

Torres, E. M., Sokolsky, T., Tucker, C. M., Chan, L. Y., Boselli, M., Dunham, M. J., & Amon, A. (2007). Effects of aneuploidy on cellular physiology and cell division in haploid yeast. Science, 317(5840), 916-924. doi: 10.1126/science.1142210

Recommended Readings: Joseph Schlessinger, Ph.D., May 13

Friday Lecture Series
Friday, May 13, 2016
3:45 p.m., Caspary Auditorium

Joseph Schlessinger, Ph.D.
William H. Prusoff Professor and Chair,
Department of Pharmacology,
Yale School of Medicine

Cell Signaling by Receptor Tyrosine Kinases: From Basic Principles to Cancer Therapy

Recommended Readings:

Empirical Articles

Bae, J. H., Lew, E. D., Yuzawa, S., Tomé, F., Lax, I., & Schlessinger, J. (2009). The selectivity of receptor tyrosine kinase signaling is controlled by a secondary SH2 domain binding site. Cell, 138(3), 514-524. doi: 10.1016/j.cell.2009.05.028.

Chung, I., Akita, R., Vandlen, R., Toomre, D., Schlessinger, J., & Mellman, I. (2010). Spatial control of EGF receptor activation by reversible dimerization on living cells. Nature, 464(7289), 783-787. doi: 10.1038/nature08827.

Yuzawa, S., Opatowsky, Y., Zhang, Z., Mandiyan, V., Lax, I., & Schlessinger, J. (2007). Structural basis for activation of the receptor tyrosine kinase KIT by stem cell factor. Cell, 130(2), 323-334.

Review Papers

Lemmon, M. A., & Schlessinger, J. (2010). Cell signaling by receptor tyrosine kinases. Cell, 141(7), 1117-1134. doi: 10.1016/j.cell.2010.06.011.

Schlessinger, J. (2014). Receptor tyrosine kinases: legacy of the first two decades. Cold Spring Harbor Perspectives in Biology, 6(3), a008912. doi: 10.1101/cshperspect.a008912.

Recommended Readings: Tom A. Rapoport, Ph.D., May 6

Friday Lecture Series
Friday, May 6, 2016
3:45 p.m., Caspary Auditorium

Tom A. Rapoport, Ph.D.
Professor, Department of Cell Biology,
Harvard Medical School
Investigator, Howard Hughes Medical Institute

How an Organelle Gets into Shape

Recommended Readings:

Hu, J., Shibata, Y., Voss, C., Shemesh, T., Li, Z., Coughlin, M., … & Prinz, W. A. (2008). Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Science, 319(5867), 1247-1250. doi: 10.1126/science.1153634.

Shemesh, T., Klemm, R. W., Romano, F. B., Wang, S., Vaughan, J., Zhuang, X., … & Rapoport, T. A. (2014). A model for the generation and interconversion of ER morphologies. Proceedings of the National Academy of Sciences, 111(49), E5243-E5251. doi: 10.1073/pnas.1419997111.

Shibata, Y., Hu, J., Kozlov, M. M., & Rapoport, T. A. (2009). Mechanisms shaping the membranes of cellular organelles. Annual Review of Cell and Developmental, 25, 329-354. doi: 10.1146/annurev.cellbio.042308.113324.

Shibata, Y., Shemesh, T., Prinz, W. A., Palazzo, A. F., Kozlov, M. M., & Rapoport, T. A. (2010). Mechanisms determining the morphology of the peripheral ER. Cell, 143(5), 774-788. doi: 10.1016/j.cell.2010.11.007.

Shibata, Y., Voeltz, G. K., & Rapoport, T. A. (2006). Rough sheets and smooth tubules. Cell, 126(3), 435-439.

Recommended Readings: Mary Jeanne Kreek, M.D., May 2

Monday Lecture Series
Monday, May 2, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Mary Jeanne Kreek, M.D.
Senior Attending Physician,
The Rockefeller University Hospital
Patrick E. and Beatrice M. Haggerty Professor and Head,
Laboratory of the Biology of Addictive Diseases,
The Rockefeller University

50th Anniversary of First Research Paper on Methadone Maintenance Treatment: Update on Molecular, Neurobiological, Behavioral, and Genetic Research

Recommended Readings:

Bond, C., LaForge, K. S., Tian, M., Melia, D., Zhang, S., Borg, L., … & Tischfield, J. A. (1998). Single-nucleotide polymorphism in the human mu opioid receptor gene alters β-endorphin binding and activity: possible implications for opiate addiction. Proceedings of the National Academy of Sciences, 95(16), 9608-9613.

Butelman, E. R., Yuferov, V., & Kreek, M. J. (2012). κ-opioid receptor/dynorphin system: genetic and pharmacotherapeutic implications for addiction. Trends in Neurosciences, 35(10), 587-596. doi:10.1016/j.tins.2012.05.005.

Dole, V. P., Nyswander, M. E., & Kreek, M. J. (1966). Narcotic blockade. Archives of Internal Medicine, 118(4), 304-309. doi:10.1001/archinte.1966.00290160004002

Kreek, M. J., Nielsen, D. A., Butelman, E. R., & LaForge, K. S. (2005). Genetic influences on impulsivity, risk taking, stress responsivity and vulnerability to drug abuse and addiction. Nature Neuroscience, 8(11), 1450-1457. doi:10.1038/nn1583

Reed, B., Butelman, E. R., Yuferov, V., Randesi, M., & Kreek, M. J. (2014). Genetics of opiate addiction. Current Psychiatry Reports, 16(11), 1-12. doi:10.1007/s11920-014-0504-6.

Recommended Readings: Tarun Kapoor, Ph.D., April 18

Monday Lecture Series
Monday, April 18, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Tarun Kapoor, Ph.D.
Pels Family Professor and Head,
Selma and Lawrence Ruben Laboratory of Chemistry and Cell Biology,
The Rockefeller University

New Chemical Probes for Ribosome Biogenesis and Cytoskeleton Organization

Recommended Readings

Kasap, C., Elemento, O., & Kapoor, T. M. (2014). DrugTargetSeqR: a genomics-and CRISPR-Cas9–based method to analyze drug targets. Nature Chemical Biology, 10(8), 626-628. doi: 10.1038/nchembio.1551

See, S. K., Hoogendoorn, S., Chung, A. H., Ye, F., Steinman, J. B., Sakata-Kato, T., … & Chen, J. K. (2015). Cytoplasmic dynein antagonists with improved potency and isoform selectivity. ACS Chemical Biology, 11(1), 53-60. doi:10.1021/acschembio.5b00895

Shimamoto, Y., Forth, S., & Kapoor, T. M. (2015). Measuring pushing and braking forces generated by ensembles of kinesin-5 crosslinking two microtubules. Developmental Cell, 34(6), 669-681. doi:10.1016/j.devcel.2015.08.017

Recommended Readings: Rod MacKinnon, M.D., Ph.D., April 11

Monday Lecture Series
Monday, April 11, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Rod MacKinnon, M.D., Ph.D.
John D. Rockefeller Jr. Professor and Head,
Laboratory of Molecular Neurobiology and Biophysics,
The Rockefeller University
Investigator, Howard Hughes Medical Institute

Some Experiments That Excite Me Right Now

Recommended Readings

Brohawn, S. G., Campbell, E. B., & MacKinnon, R. (2014). Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel. Nature, 516(7529), 126-130. doi:10.1038/nature14013

Doyle, D. A., Cabral, J. M., Pfuetzner, R. A., Kuo, A., Gulbis, J. M., Cohen, S. L., … & MacKinnon, R. (1998). The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science, 280(5360), 69-77. doi:10.1126/science.280.5360.69

Hite, R. K., Yuan, P., Li, Z., Hsuing, Y., Walz, T., & MacKinnon, R. (2015). Cryo-electron microscopy structure of the Slo2. 2 Na+-activated K+ channel. Nature, 527(7577), 198-203.  doi:10.1038/nature14958

MacKinnon, R. (2004). Potassium channels and the atomic basis of selective ion conduction (Nobel Lecture). Angewandte Chemie, 43(33), 4265-4277.