Recommended Readings: Franziska Bleichert, Ph.D., March 21

Special Lecture Series
Monday, March 21, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Franziska Bleichert, Ph.D.
Postdoctoral Fellow,
Department of Biophysics and Biophysical Chemistry,
Johns Hopkins University School of Medicine

Molucular Mechanisms for Initiating DNA Replication in Eukaryotes

Recommended Reading

Bleichert, F., Balasov, M., Chesnokov, I., Nogales, E., Botchan, M. R., & Berger, J. M. (2013). A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation. Elife, 2, e00882. doi: 10.7554/eLife.00882.

Bleichert, F., Botchan, M. R., & Berger, J. M. (2015). Crystal structure of the eukaryotic origin recognition complex. Nature. 519(7543):321-326. doi: 10.1038/nature14239

Recommended Readings: Jue Chen, Ph.D., February 27

Friday Lecture Series / Ph.D. Recruitment Lecture
Friday, February 25, 2015
3:45 p.m., Caspary Auditorium

Jue Chen, Ph.D.,
Head of Laboratory of Membrane Biology and Biophysics,
The Rockefeller University
Investigator, Howard Hughes Medical Institute

Understanding Molecular Pumps: Insights from Structural BiologyRecommended Readings

Empirical Articles

Chen, S., Oldham, M. L., Davidson, A. L., & Chen, J. (2013). Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography. Nature, 499(7458), 364-368. doi:10.1038/nature12232

Jin, M. S., Oldham, M. L., Zhang, Q., & Chen, J. (2012). Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans. Nature. 490(7421), 566-569. doi:10.1038/nature11448

Oldham, M. L., Chen, S., & Chen, J. (2013). Structural basis for substrate specificity in the Escherichia coli maltose transport system. Proceedings of the National Academy of Sciences, 110(45), 18132-18137. doi:10.1073/pnas.1311407110

Review Papers

Chen, J. (2013). Molecular mechanism of the Escherichia coli maltose transporterCurrent Opinion in Structural Biology, 23(4), 492-498. doi: 10.1016/

Recommended Readings: Angeline Lyon, Ph.D. Monday, January 6

Monday, January 6, 2014
4:00 p.m., Carson Family Auditorium

Angeline Lyon, Ph.D.
American Heart Association Postdoctoral Fellow
Life Sciences Institute, University of Michigan

Molecular Mechanisms of Phospholipase Cβ Regulation

Recommended Readings:

Empirical Articles

Hicks, S. N., Jezyk, M. R., Gershburg, S., Seifert, J. P., Harden, T. K., & Sondek, J. (2008). General and versatile autoinhibition of PLC isozymes. Molecular Cell, 31(3), 383–394. doi:10.1016/j.molcel.2008.06.018

Lyon, A. M., Dutta, S., Boguth, C. A., Skiniotis, G., & Tesmer, J. J. G. (2013). Full-length Gα(q)-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain. Nature: Structural & Molecular Biology, 20(3), 355–362. doi:10.1038/nsmb.2497

Lyon, A. M., Tesmer, V. M., Dhamsania, V. D., Thal, D. M., Gutierrez, J., Chowdhury, S., … Tesmer, J. J. G. (2011). An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation. Nature: Structural & Molecular Biology, 18(9), 999–1005. doi:10.1038/nsmb.2095

Tadano, M., Edamatsu, H., Minamisawa, S., Yokoyama, U., Ishikawa, Y., Suzuki, N., … Kataoka, T. (2005). Congenital semilunar valvulogenesis defect in mice deficient in phospholipase C epsilon. Molecular and Cellular Biology, 25(6), 2191–2199. doi:10.1128/MCB.25.6.2191-2199.2005

Wang, H., Oestreich, E. A., Maekawa, N., Bullard, T. A., Vikstrom, K. L., Dirksen, R. T., … Smrcka, A. V. (2005). Phospholipase C epsilon modulates beta-adrenergic receptor-dependent cardiac contraction and inhibits cardiac hypertrophy. Circulation Research, 97(12), 1305–13. doi:10.1161/

Review Papers

Gresset, A., Sondek, J., & Harden, T. K. (2012). The phospholipase C isozymes and their regulationSub-Cellular Biochemistry58, 61–94. doi:10.1007/978-94-007-3012-0_3

Suh, P.-G., Park, J.-I., Manzoli, L., Cocco, L., Peak, J. C., Katan, M., … Ryu, S. H. (2008). Multiple roles of phosphoinositide-specific phospholipase C isozymesBMB Reports41(6), 415–434.

Woodcock, E. a, Kistler, P. M., & Ju, Y.-K. (2009). Phosphoinositide signalling and cardiac arrhythmiasCardiovascular Research82(2), 286–295. doi:10.1093/cvr/cvn283

Structural Biology Lecture Series: Venki Ramakrishnan Ph.D. Wed Sept 28 2011

Structural Biology Lecture Series

The Structural Basis of Decoding by the Ribosome

Dr. Venki Ramakrishnan

2009 Nobelist

Medical Research Council Laboratory of Molecular Biology

Wednesday September 28, 11 am, Caspary Hall

Recommended Readings:

Voorhees RM; Schmeing TM; Kelley AC; Ramakrishnan V. 2010. The mechanism for activation of GTP hydrolysis on the ribosome. Science 330:835-838.

Schmeing TM; Voorhees RM; Kelley AC; et al.  2009. The crystal structure of the ribosome bound to EF-Tu and aminoacyl-tRNA.  Science  326(5953):688-694.

Gao Y-G; Selmer M; Dunham CM; Weixlbaumer A; Kelley AC; Ramakrishnan V. 2009. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 326:694-699.

Weixlbaumer A; Hong J; Neubauer C; Voorhees RM; Sabine P; Kelley AC; Ramakrishnan V. 2008. Insights into translational termination from the structure of RF2 bound to the ribosome. Science 322:953-956.

Selmer M; Duham CM; Murphy FV; Weixlbaumer A; Sabine P; Kelley AC; Weir JR; Ramakrishnan V. 2006. Science 313:1935-1942.

Petry S; Brodeson DE; Murphy FV; et al.  2005.  Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon.  Cell  123(7):1255-1266.

Ogle, JM; Murphy FV; Tarry MJ; et al.  2002. Selection of tRNA by the ribosome requires a transition from an open to a closed form.  Cell  111(5):721-732.

Wimberly BT; Brodeson DE; Clemons WM; et al.  Structure of the ribosomal subunit. 2000. NATURE   407(6802): 327-339.

Carter AP; Clemons WM; Broderson DE; et al.  2000.  Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics.  NATURE  407(6802):340-348.