Recommended Readings: James Wells, Ph.D., February 5

Friday Lecture Series
Friday, February 5, 2016
3:45 p.m., Carson Family Caspary Auditorium

James M. Wells, Ph.D.
Professor of Pediatrics,
Perinatal Institute Endowed Professor,
Division of Development Biology,
Director for Basic Research,
Division of Endocrinology,
Cincinnati Children’s Hospital Medical Center

Pluripotent Stem Cell-based Models of Human Gastrointestinal Development and Disease

Recommended Reading

Empirical Articles

McCracken, K. W., Catá, E. M., Crawford, C. M., Sinagoga, K. L., Schumacher, M., Rockich, B. E., … & Wells, J. M. (2014). Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature. 516(7531):400-404. doi: 10.1038/nature13863.

Spence, J. R., Mayhew, C. N., Rankin, S. A., Kuhar, M. F., Vallance, J. E., Tolle, K., … & Shroyer, N. F. (2011). Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 470(7332), 105-109. doi: 10.1038/nature09691

Watson, C. L., Mahe, M. M., Múnera, J., Howell, J. C., Sundaram, N., Poling, H. M., … & Grabowski, G. (2014). An in vivo model of human small intestine using pluripotent stem cells. Nature Medicine, 20(11), 1310-1314. doi: 10.1038/nm.3737

Review Papers

Sinagoga, K. L., & Wells, J. M. (2015). Generating human intestinal tissues from pluripotent stem cells to study development and disease. The EMBO Journal, 34(9):1149-63. doi: 10.15252/embj.201490686

Wells, J. M., & Spence, J. R. (2014). How to make an intestine. Development, 141(4), 752-760. doi: 10.1242/dev.097386.

Recommended Readings: Leonard Zon, M.D.

SPECIAL SEMINAR
Monday, November 4, 2013
4:00 p.m., Carson Family Auditorium

Leonard Zon, M.D.
Director of the Stem Cell Program at Children’s Hospital Boston
Grousbeck Professor of Pediatric Medicine, Harvard Medical School
Investigator, Howard Hughes Medical Institute

Pathways that Stimulate Stem Cell Development and Self-Renewal

Recommended Readings:

Review Papers

De Jong, J. L. O., & Zon, L. I. (2005). Use of the zebrafish system to study primitive and definitive hematopoiesis. Annual Review of Genetics, 39, 481–501. doi:10.1146/annurev.genet.39.073003.095931

Orkin, S. H., & Zon, L. I. (2008). Hematopoiesis: an evolving paradigm for stem cell biologyCell132(4), 631–644. doi:10.1016/j.cell.2008.01.025

White, R., Rose, K., & Zon, L. (2013). Zebrafish cancer: the state of the art and the path forward. Nature Reviews Cancer, 13(9), 624–636. doi:10.1038/nrc3589

Zon, L. I. (2008). Intrinsic and extrinsic control of haematopoietic stem-cell self-renewal. Nature, 453(7193), 306–313. doi:10.1038/nature07038

Empirical Articles

Goessling, W., North, T. E., Loewer, S., Lord, A. M., Lee, S., Stoick-Cooper, C. L., … Zon, L. I. (2009). Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration. Cell, 136(6), 1136–1147. doi:10.1016/j.cell.2009.01.015

Lengerke, C., Schmitt, S., Bowman, T. V, Jang, I. H., Maouche-Chretien, L., McKinney-Freeman, S., … Daley, G. Q. (2008). BMP and Wnt specify hematopoietic fate by activation of the Cdx-Hox pathway. Cell Stem Cell, 2(1), 72–82. doi:10.1016/j.stem.2007.10.022

North, T. E., Goessling, W., Peeters, M., Li, P., Ceol, C., Lord, A. M., … Zon, L. I. (2009). Hematopoietic stem cell development is dependent on blood flow. Cell, 137(4), 736–748. doi:10.1016/j.cell.2009.04.023

North, T. E., Goessling, W., Walkley, C. R., Lengerke, C., Kopani, K. R., Lord, A. M., … Zon, L. I. (2007). Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis. Nature, 447(7147), 1007–1011. doi:10.1038/nature05883

Understanding metastasis: Collective Cell Migration Controlled by Wnt and Fgf Signaling

In the November 11, 2008 issue of Developmental Cell, investigators from the University of Utah School of Medicine report the results of their studies in an article entitled Wnt/B-Catenin and Fgf Signaling Control Collective Cell Migration by Restricting Chemokine Receptor Expression.  These studies demonstrate a link between Wnt and Fgf signaling pathways in zebrafish and their impact on collective cell migration. 

The Wnt pathway regulates cell-to-cell communication in embryogenesis and cancer and Fgf influences embryongenesis, healing, and cell proliferation.  Piotrowski and Aman demonstrate for the first time that the interaction between Wnt and Fgf pathways is critical for collective cell migration.  Each pathway can restrict chemokine receptor expression and thereby elucidate how some types of cancer metastisize. 

Extracted from Developmental Cell and ScienceDaily.