Recommended Readings: Robert McGinty, M.D., Ph.D., February 18th

Special Lecture,
Wednesday, February 18th, 2015
4:00 p.m., Carson Family Auditorium (CRC)

Robert McGinty, M.D., Ph.D.
Damon Runyun Postdoctoral Fellow,
Dale F. Frey Breakthrough Scientist,
Department of Biochemistry & Molecular Biology,
The Pennsylvania State University

Chemistry, Crystallography and Nucleosome Ubiquitylation

Recommended Readings

Empirical Articles

Kim, J., Kim, J., McGinty, R. K., Nguyen, U. T., Muir, T. W., Allis, C. D., & Roeder, R. G. (2013). The n-SET domain of Set1 regulates H2B ubiquitylation-dependent H3K4 methylation. Molecular Cell, 49(6), 1121-1133. doi:10.1016/j.molcel.2013.01.034

McGinty, R. K., Henrici, R. C., & Tan, S. (2014). Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome. Nature, 514(7524), 591-596. doi:10.1038/nature13890

Tang, Z., Chen, W. Y., Shimada, M., Nguyen, U. T., Kim, J., Sun, X. J., … & Roeder, R. G. (2013). SET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53. Cell, 154(2), 297-310. doi:10.1016/j.cell.2013.06.027

Review Papers

McGinty, R. K., & Tan, S. (In Press). Nucleosome structure and function. Chemical Reviews. doi:10.1021/cr500373h

Recommended Readings: Tom W. Muir, Ph.D.

Friday Lecture Series

A Synthetic “Kiss of Death”

 Tom W. Muir, Ph.D.

Richard E. Salomon Professor and head, Selma and Lawrence Ruben Laboratory of Synthetic Protein Chemistry

The Rockefeller University

March 6, 2009

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Articles:

Alexeev, D., P. N. Barlow, S. M. Bury, J. -D Charrier, A. Cooper, D. Hadfield, C. Jamieson, et al. 2003. Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core. ChemBioChem. 4(9):894-896.


Alexeev, D., S. M. Bury, M. A. Turner, O. M. Ogunjobi, T. W. Muir, R. Ramage, and L. Sawyer. 1994. Synthetic, structural and biological studies of the ubiquitin system: Chemically synthesized and native ubiquitin fold into identical three-dimensional structures. Biochemical Journal. 299(1):159-163.


Chatterjee, C., R. K. McGinty, J. -P Pellois, and T. W. Muir. 2007. Auxiliary-mediated site-specific peptide ubiquitylation. Angewandte Chemie – International Edition. 46(16):2814-2818.


Chiang, K. P., and T. W. Muir. 2008. Systems- and molecular-level elucidation of signaling processes through chemistry. Science signaling. 1(42):pp. pe45.


Layfield, R., K. Franklin, M. Landon, G. Walker, P. Wang, R. Ramage, A. Brown, et al. 1999. Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes. Analytical Biochemistry. 274(1):40-49.


Love, S. G., T. W. Muir, R. Ramage, K. T. Shaw, D. Alexeev, L. Sawyer, S. M. Kelly, et al. 1997. Synthetic, structural and biological studies of the ubiquitin system: Synthesis and crystal structure of an analogue containing unnatural amino acids. Biochemical Journal. 323(3):727-734.


McGinty, R. K., J. Kim, C. Chatterjee, R. G. Roeder, and T. W. Muir. 2008. Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation. Nature. 453(7196):812-816.


Ramage, R., J. Green, T. W. Muir, O. M. Ogunjobi, S. Love, and K. Shaw. 1994. Synthetic, structural and biological studies of the ubiquitin system: The total chemical synthesis of ubiquitin. Biochemical Journal. 299(1):151-158.