Recommended Readings: Jennifer Doudna, Ph.D. September 19

Friday Lecture Series
Friday, September 19, 2014
3:45 p.m., Caspary Auditorium

Jennifer Doudna, Ph.D.
Li Ka Shing Chancellor’s Chair in Biomedical and Health Sciences,
Professor of Biochemistry, Biophysics and Structural Biology,
Departments of Molecular and Cell Biology and Chemistry,
University of California, Berkeley
Investigator, Howard Hughes Medical Institute

CRISPR Systems: A Revolution in Basic Science and Biotechnology

Recommended Readings

Empirical Articles

Haurwitz, R. E., Jinek, M., Wiedenheft, B., Zhou, K., & Doudna, J. a. (2010). Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Science, 329(5997), 1355–1358. doi:10.1126/science.1192272

Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C., & Doudna, J. a. (2014). DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature, 507(7490), 62–67. doi:10.1038/nature13011

Wiedenheft, B., Sternberg, S. H., & Doudna, J. a. (2012). RNA-guided genetic silencing systems in bacteria and archaea. Nature, 482(7385), 331–338. doi:10.1038/nature10886

Review Papers

Bhaya, D., Davison, M., & Barrangou, R. (2011). CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. Annual Review of Genetics, 45, 273–97. doi:10.1146/annurev-genet-110410-132430

Hsu, P. D., Lander, E. S., & Zhang, F. (2014). Development and Applications of CRISPR-Cas9 for Genome Engineering. Cell, 157(6), 1262–1278. doi:10.1016/j.cell.2014.05.010

Recommended Readings: Mario Halic, PhD Oct 11 2010

Monday Lecture Series

Dicer-independent Primal RNAs Trigger RNAi and Heterochromatin Formation

Mario Halic, Ph.D.,

Post Doctoral Fellow 

Department of  Cell Biology

Harvard Medical School

October 11, 2010

4:00 p.m.  Welch Hall Level Two


Recommended Readings:

Yang JS, Maurin T, Robine N, et al.  2010.   Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis.   PNAS   107(34):15163-15168   

Lee HC, Li LD, Gu WF, et al.  2010.  Diverse Pathways Generate MicroRNA-like RNAs and Dicer-Independent Small Interfering RNAs in Fungi.  MOLECULAR CELL   38 ( 6):803-814   

Cifuentes D, Xue HL, Taylor DW, et al.  2010.  A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity.   SCIENCE    328( 5986):1694-1698   

 Cheloufi S, Dos Santos CO, Chong MMW, et al.: 2010.  A Dicer-independent miRNA biogenesis pathway that requires Ago catalysis.   NATURE   465( 7298):584-

Halic M, Moazed D.  2010.   Dicer-Independent Primal RNAs Trigger RNAi and Heterochromatin Formation. CELL    140( 4):504-516  

Cao F, Li XZ, Hiew S, et al.  2009.  Dicer independent small RNAs associate with telomeric heterochromatin.  RNA-A PUBLICATION OF THE RNA SOCIETY    15(7):1274-1281.   Please request from Markus Library.

Kawamura Y, Saito K, Kin T, et al.  2008.  Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells.   NATURE   453(7196):793-U5

Klattenhoff C, Theurkauf W .  2008.  Biogenesis and germline functions of piRNAs.  DEVELOPMENT  135(1):3-