Recommended Readings: Edward H. Egelman, Ph.D. Friday January 19th, 2018

Friday Lectures

Friday, January 19, 2018  3:45 p.m.

Caspary Auditorium

Edward Egelman, Ph.D.

Harrison Distinguished Professor

Biochemistry and Molecular Genetics

University of Virginia Medical School

Cryo-EM of Protein and Nucleoprotein Polymers at Near-Atomic Resolution

Recommended Readings:

Science News

Kenny Walter. Virus Holds Key to Durable Material. (2017, Jul 20). Virus Holds Key to Durable Material. R&D Magazine

Empirical Articles

Egelman, Edward H. (2017). Cryo-EM of bacterial pili and archaeal flagellar filaments. CURRENT OPINION IN STRUCTURAL BIOLOGY. 46: 31-37

Wang, Fengbin; Burrage, Andrew M.; Postel, Sandra; et al. (2017). A structural model of flagellar filament switching across multiple bacterial species. NATURE COMMUNICATIONS. 8: 960

Wang, Fengbin; Coureuil, Mathieu; Osinski, Tomasz; et al. (2017). Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution. STRUCTURE. 25 (9): 1423-+

Kasson, Peter; DiMaio, Frank; Yu, Xiong; et al. (2017). Model for a novel membrane envelope in a filamentous hyperthermophilic virus. ELIFE. 6  

Kolappan, Subramania; Coureuil, Mathieu; Yu, Xiong; et al. (2016). Structure of the Neisseria meningitidis Type IV pilus. NATURE COMMUNICATIONS. 7: 13015

Braun, Tatjana; Vos, Matthijn R.; Kalisman, Nir; et al. (2016). Archaeal flagellin combines a bacterial type IV pilin domain with an Ig-like domain. PNAS. 113 (37): 10352-10357

Egelman, Edward H. (2016). The Current Revolution in Cryo-EM. BIOPHYSICAL JOURNAL. 110 (5): 1008-1012

Wang, Ray Yu-Ruei; Kudryashev, Mikhail; Li, Xueming; et al. (2015). De novo protein structure determination from near-atomic-resolution cryo-EM maps. NATURE METHODS. 12 (4): 335-U84

DiMaio, Frank; Yu, Xiong; Rensen, Elena; et al. (2015). A virus that infects a hyperthermophile encapsidates A-form DNA. SCIENCE. 348 (6237): 914-917

Review Papers

Egelman, Edward H. (2015). Three-dimensional reconstruction of helical polymers. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 581: 54-58

Recommended Readings: Eva Nogales, Ph.D., February 20

Friday Lecture Series
Friday, February 20, 2015
3:45 p.m., Caspary Auditorium

Eva Nogales, Ph.D.
Professor of Biochemistry, Biophysics and Structural Biology,
University of California, Berkeley
Senior Faculty Scientist, Life Sciences Division,
Lawrence Berkeley National Laboratory
Investigator, Howard Hughes Medical Institute

Cryo-EM Studies of the Human Transcription Initiation Machinery

Recommended Readings

Cianfrocco, M. a, & Nogales, E. (2013). Regulatory interplay between TFIID’s conformational transitions and its modular interaction with core promoter DNA. Transcription, 4(3), 1–7. doi:10.4161/trns.25291

Cianfrocco, M. A., Kassavetis, G. A., Grob, P., Fang, J., Juven-Gershon, T., Kadonaga, J. T., & Nogales, E. (2013). Human TFIID binds to core promoter DNA in a reorganized structural state. Cell, 152(1-2), 120–131. doi:10.1016/j.cell.2012.12.005

He, Y., Fang, J., Taatjes, D. J., & Nogales, E. (2013). Structural visualization of key steps in human transcription initiation. Nature, 495(7442), 481–486. doi:10.1038/nature11991

Recommended Readings: Roland Beckmann, Ph.D. Monday, Nov 25

SPECIAL SEMINAR
Monday, November 25, 2013
4:00 p.m., Carson Family Auditorium

Roland Beckmann, Ph.D.
Professor
Chair of Cellular Biochemistry
Gene Center Munich
Ludwig Maximilian University of Munich

Cryo-EM of ribosomal particles:
From ribosome biogenesis to protein translocation and quality control

Recommended Readings:

Empirical Articles

Anger, A. M., Armache, J.-P., Berninghausen, O., Habeck, M., Subklewe, M., Wilson, D. N., & Beckmann, R. (2013). Structures of the human and Drosophila 80S ribosome. Nature, 497(7447), 80–85. doi:10.1038/nature12104

Armache, J.-P., Jarasch, A., Anger, A. M., Villa, E., Becker, T., Bhushan, S., … Beckmann, R. (2010). Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proceedings of the National Academy of Sciences of the United States of America, 107(46), 19748–19753. doi:10.1073/pnas.1009999107

Beckmann, R., Spahn, C. M., Eswar, N., Helmers, J., Penczek, P. a, Sali, a, … Blobel, G. (2001). Architecture of the protein-conducting channel associated with the translating 80S ribosome. Cell, 107(3), 361–372.

Ben-Shem, A., Garreau de Loubresse, N., Melnikov, S., Jenner, L., Yusupova, G., & Yusupov, M. (2011). The structure of the eukaryotic ribosome at 3.0 Å resolution. Science, 334(6062), 1524–1529. doi:10.1126/science.1212642

Leidig, C., Bange, G., Kopp, J., Amlacher, S., Aravind, A., Wickles, S., … Sinning, I. (2013). Structural characterization of a eukaryotic chaperone–the ribosome-associated complex. Nature structural & molecular biology, 20(1), 23–28. doi:10.1038/nsmb.2447

Review Papers

Klinge, S., Voigts-Hoffmann, F., Leibundgut, M., & Ban, N. (2012). Atomic structures of the eukaryotic ribosome. Trends in biochemical sciences, 37(5), 189–198. doi:10.1016/j.tibs.2012.02.007

Granneman, S., & Baserga, S. J. (2004). Ribosome biogenesis: of knobs and RNA processing. Experimental cell research, 296(1), 43–50. doi:10.1016/j.yexcr.2004.03.016

Schmeing, T. M., & Ramakrishnan, V. (2009). What recent ribosome structures have revealed about the mechanism of translation. Nature, 461(7268), 1234–1242. doi:10.1038/nature08403