Recommended Readings: Emmanuelle Charpentier, Ph.D., January 22

Friday Lecture Series
Friday, January 22, 2016
3:45 p.m., Caspary Auditorium

Emmanuelle Charpentier, Ph.D.
Professor, Department of Regulation in Infection Biology
Director, Max Planck Institute for Infection Biology;
Visiting Professor, Laboratory for Molecular Infection Medicine Sweden, Umeå University

The Transformative Genome Engineering Technology CRISPR-Cas9: Lessons Learned from bacteria

Recommended Reading

Empirical Articles

Chylinski, K., Le Rhun, A., & Charpentier, E. (2013). The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems. RNA Biology, 10(5), 726-737. doi:10.4161/rna.24321

Fonfara, I., Le Rhun, A., Chylinski, K., Makarova, K. S., Lécrivain, A. L., Bzdrenga, J., … & Charpentier, E. (2014). Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems. Nucleic acids research, 42(4), 2577-2590. doi:10.1093/nar/gkt1074.

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012). A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science, 337(6096), 816-821. doi:10.1126/science.1225829.

Review Papers

Charpentier, E., Richter, H., van der Oost, J., & White, M. F. (2015). Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity. FEMS Microbiology Reviews, 39(3), 428-441. doi:10.1093/femsre/fuv023.

Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014). Classification and evolution of type II CRISPR-Cas systems. Nucleic acids research, 42(10), 6091-6105. doi:10.1093/nar/gku241

Recommended Readings: Luciano Marraffini, Ph.D., October 9th

Friday Lecture Series
Friday, October 9th, 2015
3:45 p.m., Caspary Auditorium

Luciano Marraffini, Ph.D.
Assistant Professor and Head,
Laboratory of Bacteriology,
The Rockefeller University

CRISPR-Cas: The Adaptive Immune System of Prokaryotes

Recommended Readings

Empirical Articles

Goldberg, G. W., Jiang, W., Bikard, D., & Marraffini, L. A. (2014). Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting. Nature, 514(7524), 633-637. doi:10.1038/nature13637.

Heler, R., Samai, P., Modell, J. W., Weiner, C., Goldberg, G. W., Bikard, D., & Marraffini, L. A. (2015). Cas9 specifies functional viral targets during CRISPR-Cas adaptation. Nature, 19(7542), 199-202. doi:10.1038/nature14245.

Jiang, W., Maniv, I., Arain, F., Wang, Y., Levin, B. R., & Marraffini, L. A. (2012). Dealing with the evolutionary downside of CRISPR immunity: bacteria and beneficial plasmids. PLoS Genetics, 9(9), e1003844-e1003844. doi:10.1371/journal.pgen.1003844.

Review Papers

Barrangou, R., & Marraffini, L. A. (2014). CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity. Molecular Cell, 54(2), 234-244. doi:10.1016/j.molcel.2014.03.011.

Hatoum-Aslan, A., & Marraffini, L. A. (2014). Impact of CRISPR immunity on the emergence and virulence of bacterial pathogens. Current Opinion in Microbiology, 17, 82-90. doi:10.1016/j.mib.2013.12.001

Recommended Readings: Rudolf Jaenisch, M.D. December 12

Friday Lecture Series
Friday, December 12, 2014
3:45 p.m., Caspary Auditorium

Rudolf Jaenisch, M.D.
Professor of Biology,
Massachusetts Institute of Technology
Whitehead Institute for Biomedical Research

iPS Cell Technology, Gene Editing and Disease Research

Recommended Readings

Empirical Articles

Chung, C. Y., Khurana, V., Auluck, P. K., Tardiff, D. F., Mazzulli, J. R., Soldner, F., … Lindquist, S. (2013). Identification and rescue of α-synuclein toxicity in Parkinson patient-derived neurons. Science, 342(6161), 983–987. doi:10.1126/science.1245296

Li, Y., Wang, H., Muffat, J., Cheng, A. W., Orlando, D. a, Lovén, J., … Jaenisch, R. (2013). Global transcriptional and translational repression in human-embryonic-stem-cell-derived Rett syndrome neurons. Cell Stem Cell, 13(4), 446–458. doi:10.1016/j.stem.2013.09.001

Soldner, F., Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan, R., … Jaenisch, R. (2011). Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell, 146(2), 318–331. doi:10.1016/j.cell.2011.06.019

Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013). One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell, 153(4), 910–918. doi:10.1016/j.cell.2013.04.025

Review Papers

Buganim, Y., Faddah, D. A., & Jaenisch, R. (2013). Mechanisms and models of somatic cell reprogramming. Nature Reviews Genetics, 14(6), 427–439. doi:10.1038/nrg3473

Theunissen, T. W., & Jaenisch, R. (2014). Molecular Control of Induced Pluripotency. Cell Stem Cell, 14(6), 720–734. doi:10.1016/j.stem.2014.05.002