Recommended Readings: Ying Lu, Ph.D., February 29

Special Lecture Series
Monday, February 29
4:00 p.m., Carson Family Auditorium (CRC)

Ying Lu, Ph.D.,
Postdoctoral Fellow,
Department of Systems Biology,
Harvard Medical School

Specificity, Efficiency and Processivity in the Eukaryotic Protein Degradation System

Recommended Readings

Lu, Y., Lee, B. H., King, R. W., Finley, D., & Kirschner, M. W. (2015). Substrate degradation by the proteasome: A single-molecule kinetic analysis. Science, 348(6231), 1250834. doi: 10.1126/science.1250834.

Lu, Y., Wang, W., & Kirschner, M. W. (2015). Specificity of the anaphase-promoting complex: A single-molecule study. Science, 348(6231), 1248737. doi: 10.1126/science.1248737

Recommended Readings: Tom W. Muir, Ph.D.

Friday Lecture Series

A Synthetic “Kiss of Death”

 Tom W. Muir, Ph.D.

Richard E. Salomon Professor and head, Selma and Lawrence Ruben Laboratory of Synthetic Protein Chemistry

The Rockefeller University

March 6, 2009

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Articles:

Alexeev, D., P. N. Barlow, S. M. Bury, J. -D Charrier, A. Cooper, D. Hadfield, C. Jamieson, et al. 2003. Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core. ChemBioChem. 4(9):894-896.


Alexeev, D., S. M. Bury, M. A. Turner, O. M. Ogunjobi, T. W. Muir, R. Ramage, and L. Sawyer. 1994. Synthetic, structural and biological studies of the ubiquitin system: Chemically synthesized and native ubiquitin fold into identical three-dimensional structures. Biochemical Journal. 299(1):159-163.


Chatterjee, C., R. K. McGinty, J. -P Pellois, and T. W. Muir. 2007. Auxiliary-mediated site-specific peptide ubiquitylation. Angewandte Chemie – International Edition. 46(16):2814-2818.


Chiang, K. P., and T. W. Muir. 2008. Systems- and molecular-level elucidation of signaling processes through chemistry. Science signaling. 1(42):pp. pe45.


Layfield, R., K. Franklin, M. Landon, G. Walker, P. Wang, R. Ramage, A. Brown, et al. 1999. Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes. Analytical Biochemistry. 274(1):40-49.


Love, S. G., T. W. Muir, R. Ramage, K. T. Shaw, D. Alexeev, L. Sawyer, S. M. Kelly, et al. 1997. Synthetic, structural and biological studies of the ubiquitin system: Synthesis and crystal structure of an analogue containing unnatural amino acids. Biochemical Journal. 323(3):727-734.


McGinty, R. K., J. Kim, C. Chatterjee, R. G. Roeder, and T. W. Muir. 2008. Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation. Nature. 453(7196):812-816.


Ramage, R., J. Green, T. W. Muir, O. M. Ogunjobi, S. Love, and K. Shaw. 1994. Synthetic, structural and biological studies of the ubiquitin system: The total chemical synthesis of ubiquitin. Biochemical Journal. 299(1):151-158.