NIH Awards Microbiome Grants for Whole Genome Amplification of Single Cells

The National Institutes of Health this week awarded grants to a pair of technologies designed to enable whole-genome amplification of single cells for downstream analysis by sequencing as part of the Human Microbiome Project.

The first grant, worth $538,000 over two years and awarded to researchers from GE Global Research, will support the development of a whole-genome DNA amplification method based on multiple displacement amplification that will enable high-throughput DNA sequencing of entire genomes from single cells, eliminating the requirement to purify and culture each isolate, the grant’s abstract states.

The second grant, good for $440,000 over two years, will enable Stanford University scientists to develop an approach for plating, selecting, and amplifying whole genomic DNA from individual microbial cells in a hydrogel matrix, according to the grant’s abstract.

And although the two grants collectively represent a little under $1 million of the approximately $42 million worth of grants recently awarded by the NIH for human microbiome research, they may help address many of the inherent technical difficulties of isolating, amplifying, and sequencing genetic material from microorganisms that colonize various parts of the human body.

Recommended Readings: Gizem Donmez, PhD Sept 27, 2010

Monday Lecture Series

The Role of SIRT1 in Neurodegenerative Diseases

Gizem Donmez, Ph.D.,

Post Doctoral Fellow 

Glenn Laboratory for the Science of Aging, Department of Biology

Massachusetts Institute of Technology

September 27, 2010

4:00 p.m.  Welch Hall Level Two

Recommended Review Article:

Haigis MC, Sinclair DA.  2010.   Mammalian Sirtuins: Biological Insights and Disease Relevance.

Recommended Readings:

Donmez G, Wang D, Cohen DE, et al.  2010. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10.    CELL    142 ( 2):320-332   

Albani D, Polito L, Forloni G. 2010.  Sirtuins as Novel Targets for Alzheimer’s Disease and Other Neurodegenerative Disorders: Experimental and Genetic Evidence.  JOURNAL OF ALZHEIMERS DISEASE 19(1): 11-26    PLEASE CONTACT MARKUS LIBRARY FOR A COPY OF THIS ARTICLE.

Gao J, Wang WY, Mao YW, et al.   2010.  A novel pathway regulates memory and plasticity via SIRT1 and miR-134NATURE    466( 7310): 1105-U120   

Michan S, Li Y, Chou MMH, et al.   2010.  SIRT1 Is Essential for Normal Cognitive Function and Synaptic Plasticity.   JOURNAL OF NEUROSCIENCE    30(29): 9695-9707   

Tang BL, Chua CEL.  2010.  Is systemic activation of Sirt1 beneficial for ageing-associated metabolic disorders?   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS    391(1): 6-10

Tang BL.  2009.  Sirt1’s Complex Roles in Neuroprotection.   CELLULAR AND MOLECULAR NEUROBIOLOGY    29(8):1093-1103   


Scientists Develop New Method to Identify Glycosylated Proteins

Scientists of the Max Planck Institute of Biochemistry in Martinsried, Germany, have been able to identify more than 6,000 glycosylated protein sites in different tissues using a new method that peers into post-translational protein modification.  Read about this development in Cell.

Rockefeller Science Among the “Hottest” in New Research According to SCOPUS Database

Elsevier tracks activity in its SCOPUS database, a comprehensive compilation of citation information covering global science.   In their latest report of the 25 “hottest” titles in Biochemsitry, Genetics, and Molecular Biology they list a recent publication co-authored by Rockefeller’s Michel Nussenzweig.    The paper, “53BP1 Inhibits Homologous Recombination in Brca1-Deficient Cells by Blocking Resection of DNA Breaks  was published in the April issue of Cell.   They also cite among the top 25 an article reporting research from the de Lange lab:  “Persistent Telomere Damage Induces Bypass of Mitosis and Tetraploidy ” also published in Cell in April.