Recommended Readings: Pascale Cossart, Ph.D.

Friday Lecture Series, Ernst A.H. Friedheim Memorial Lecture

The Bacterial Pathogen Listeria monocytogenes: An Amazing Multifaceted Model

Pascale Cossart, Ph.D.

Head, bacteria-cell interactions unit, Director, INSERM unit, Director, Department of cell biology and infection, and Professor, Pasteur Institute

International Research Scholar, Howard Hughes Medical Institute

Friday, January 23, 2009

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Articles:

Bierne, H., and P. Cossart. 2007. Listeria monocytogenes surface proteins: From genome predictions to function. Microbiology and Molecular Biology Reviews. 71 (2):377-397.

Cabanes, D., P. Dehoux, O. Dussurget, L. Frangeul, and P. Cossart. 2002. Surface proteins and the pathogenic potential of Listeria monocytogenes. Trends in Microbiology. 10(5):238-245.

Cossart, P. 2001. Molecular and cellular basis of the infection by Listeria monocytogenes: An overview. International Journal of Medical Microbiology. 291(6-7):401-409.

Cossart, P., and H. Bierne. 2001. The use of host cell machinery in the pathogenesis of Listeria monocytogenes. Current Opinion in Immunology. 13(1):96-103.

Cossart, P., J. Pizarro-Cerda, and M. Lecuit. 2003. Invasion of mammalian cells by Listeria monocytogenes: Functional mimicry to subvert cellular functions. Trends in Cell Biology. 13(1):23-31.

Dussurget, O., Pizarro-Cerda, J., and Cossart, P. 2004. Molecular determinants of Listeria monocytogenes virulence. Annual Review of Microbiology. 58:587-610.

Hamon, M., H. Bierne, and P. Cossart. 2006. Listeria monocytogenes: A multifaceted model. Nature Reviews Microbiology. 4(6):423-434.

Pizarro-Cerda, J., and P. Cossart. 2006. Bacterial adhesion and entry into host cells. Cell. 124(4):715-727.

Pizarro-Cerda, J., and P. Cossart. 2006. Subversion of cellular functions by Listeria monocytogenes. Journal of Pathology. 208(2):215-223.

Recommended Readings: Edwin Ferguson, Ph.D.

Special Seminar Series: Evolution & Developmental Biology

Niche-associated Polarization of a Stem Cell in Drosophila

Edwin Ferguson, Ph.D.

Associate Professor, Department of Molecular Genetics and Cell Biology

The University of Chicago

Wednesday, January 21, 2009

4:00 p.m.-5:00 p.m. (Refreshments, 3:45 p.m.)

Second Floor, Welch Hall

Recommended Articles:

Casanueva, M. O., and E. L. Ferguson. 2004. Germline stem cell number in the Drosophila ovary is regulated by redundant mechanisms that control dpp signaling. Development. 131(9):1881-1890.

Bolivar, J., J. Pearson, L. Lopez-Onieva, and A. Gonzalez-Reyes. 2006. Genetic dissection of a stem cell niche: The case of the Drosophila ovary. Developmental Dynamics. 235(11):2969-2979.

Jin, Z., D. Kirilly, C. Weng, E. Kawase, X. Song, S. Smith, J. Schwartz, and T. Xie. 2008. Differentiation-defective stem cells outcompete normal stem cells for niche occupancy in the Drosophila ovary. Cell Stem Cell. 2(1):39-49.

Kirilly, D., and T. Xie. 2007. The Drosophila ovary: An active stem cell community. Cell Research. 17(1):15-25.

Wang, L., Z. Li, and Y. Cai. 2008. The JAK/STAT pathway positively regulates DPP signaling in the Drosophila germline stem cell niche. Journal of Cell Biology. 180(4):721-728.

Dansereau, D. A., and P. Lasko. 2008. RanBPM regulates cell shape, arrangement, and capacity of the female germline stem cell niche in Drosophila melanogaster. Journal of Cell Biology. 182(5):963-977.

Kirilly, D., E. P. Spana, N. Perrimon, R. W. Padgett, and T. Xie. 2005. BMP signaling is required for controlling somatic stem cell self-renewal in the Drosophila ovary. Developmental Cell. 9(5):651-662.

Song, X., M. D. Wong, E. Kawase, R. Xi, B. C. Ding, J. J. McCarthy, and T. Xie. 2004. Bmp signals from niche cells directly repress transcription of a differentiation-promoting gene, bag of marbles, in germline stem cells in the Drosophila ovary. Development. 131(6):1353-1364.


Recommended Readings: Agata Smogorzewska, M.D., Ph.D.

Lecture Series

When Watson and Crick Get Linked: The Fanconi Anemia Pathway and Crosslink

Agata Smogorzewska, M.D., Ph.D.

Research Fellow, Department of Pathology

Massachusetts General Hospital, Harvard Medical School

Tuesday, January 20, 2009

4:00 p.m.-5:00 p.m. (Refreshments, 3:45 p.m.)

Second Floor, Welch Hall

Recommended Articles:

Ishiai, M., H. Kitao, A. Smogorzewska, J. Tomida, A. Kinomura, E. Uchida, A. Saberi, et al. 2008. FANCI phosphorylation functions as a molecular switch to turn on the fanconi anemia pathway. Nature Structural and Molecular Biology. 15(11):1138-1146.

Smogorzewska, A., S. Matsuoka, P. Vinciguerra, E. R. McDonald III, K. E. Hurov, J. Luo, B. A. Ballif, et al. 2007. Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. Cell. 129(2):289-301.

McCabe, K. M., A. Hemphill, Y. Akkari, P. M. Jakobs, D. Pauw, S. B. Olson, R. E. Moses, and M. Grompe. 2008. ERCC1 is required for FANCD2 focus formation. Molecular Genetics and Metabolism. 95(1-2):66-73.

Tsuchida, K., and K. Komatsu. 2008. Impaired removal of DNA interstrand cross-link in nijmegen breakage syndrome and fanconi anemia, but not in BRCA-defective group. Cancer Science. 99(11):2238-2243.

Wang, W. 2008. A major switch for the fanconi anemia DNA damage-response pathway. Nature Structural and Molecular Biology. 15(11):1128-1130

Sims, A. E., E. Spiteri, R. J. Sims III, A. G. Arita, F. P. Lach, T. Landers, M. Wurm, et al. 2007. FANCI is a second monoubiquitinated member of the fanconi anemia pathway. Nature Structural and Molecular Biology. 14(6):564-567.

Bogliolo, M., O. Cabre, E. Calle, V. Castillo, A. Creus, R. Marcos, and J. Surrallés. 2002. The fanconi anaemia genome stability and tumour suppressor network. Mutagenesis. 17(6):529-538.

Grompe, M., and A. D’Andrea. 2001. Fanconi anemia and DNA repair. Human Molecular Genetics. 10(20):2253-2259.

Mathew, C. G. 2006. Fanconi anaemia genes and susceptibility to cancer. Oncogene. 25(43):5875-5884.

Niedernhofer, L. J., A. S. Lalai, and J. H. J. Hoeijmakers. 2005. Fanconi anemia (cross)linked to DNA repair. Cell. 123(7):1191-1198.

Recommended Readings: Naama Barkai, Ph.D.

Special Seminar Series: Systems Biology

Scaling in Embryonic Development

Naama Barkai, Ph.D.

Associate Professor, Department of Molecular Genetics

Weizmann Institue of Science

Wednesday, January 14, 2009

4:00 p.m.-5:00 p.m. (Refreshments, 3:45 p.m.)

Second Floor, Welch Hall

Recommended Articles:

Ambrosio, A. L., V. F. Taelman, H. X. Lee, C. A. Metzinger, C. Coffinier, and E. M. De Robertis. 2008. Crossveinless-2 is a BMP feedback inhibitor that binds Chordin/BMP to regulate xenopus embryonic patterning. Developmental Cell. 15(2):248-260.

Lewis, J. 2008. From signals to patterns: Space, time, and mathematics in developmental biology. Science. 322(5900):399-403.

Ben-Zvi, D., B. -Z Shilo, A. Fainsod, and N. Barkai. 2008. Scaling of the BMP activation gradient in Xenopus embryos. Nature. 453(7199):1205-1211.

Cooke, J. 1981. Scale of body pattern adjusts to available cell number in amphibian embryos. Nature. 290(5809):775-778.

De Robertis, E. M., and Kuroda, H. Dorsal-ventral patterning and neural induction in Xenopus embryos. Annual Review of Cell and Developmental Biology. 20:285-308.

Dosch, R., and C. Niehrs. 2000. Requirement for anti-dorsalizing morphogenetic protein in organizer patterning. Mechanisms of Development. 90(2):195-203.

Ferguson, E. L. 1996. Conservation of dorsal ventral patterning in arthropods and chordates. Current Opinion in Genetics and Development. 6(4):424-431.

Khokha, M. K., J. Yeh, T. C. Grammer, and R. M. Harland. 2005. Depletion of three BMP antagonists from Spemann’s Organizer leads to a catastrophic loss of dorsal structures. Developmental Cell. 8(3):401-411.

Related Readings: Dorothea Fiedler, Ph.D.

Lecture Series

A Genetic Map of the S. cerevisiae Phosphorylation Network

Dorothea Fiedler, Ph.D.

Post-Doctoral Fellow, Department of Cellular and Molecular Pharmacology

University of California, San Francisco

Monday, January 12, 2009

4:00 p.m.-5:00 p.m. (Refreshments, 3:45 p.m.)

Second Floor, Welch Hall

Related Articles:

Tamgüney, T., C. Zhang, D. Fiedler, K. Shokat, and D. Stokoe. 2008. Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells. Experimental Cell Research. 314(11-12):2299-2312.

Wang, J., Z. A. Knight, D. Fiedler, O. Williams, K. M. Shokat, and D. Pearce. 2008. Activity of the p110-α subunit of phosphatidylinositol-3-kinase is required for activation of epithelial sodium transport. American Journal of Physiology – Renal Physiology. 295(3):F843-F850.

Recommended Readings: Patricia Champion, Ph.D.

Lecture Series

The ESX-1 Secretion System: Substrate Recognition and Host Cell Interactions

Patricia Champion, Ph.D.

Post-Doctoral Fellow, Department of Microbiology and Immunology

University of California, San Francisco

Wednesday, January 7, 2009

4:00 p.m.-5:00 p.m. (Refreshments, 3:45 p.m.)

Second Floor, Welch Hall

Recommended Articles:

Woodworth, J. S., S. M. Fortune, and S. M. Behar. 2008. Bacterial protein secretion is required for priming of CD8+ T cells specific for the mycobacterium tuberculosis antigen CFP10. Infection and Immunity. 76(9):4199-4205.

Xu, J., O. Laine, M. Masciocchi, J. Manoranjan, J. Smith, S. J. Du, N. Edwards, X. Zhu, C. Fenselau, and L. -Y Gao. 2007. A unique mycobacterium ESX-1 protein co-secretes with CFP-10/ESAT-6 and is necessary for inhibiting phagosome maturation. Molecular Microbiology. 66(3):787-800.

Abdallah, A. M., N. C. Gey van Pittius, P. A. DiGiuseppe Champion, J. Cox, J. Luirink, C. M. J. E. Vandenbroucke-Grauls, B. J. Appelmelk, and W. Bitter. 2007. Type VII secretion – mycobacteria show the way. Nature Reviews Microbiology. 5(11):883-891.

McLaughlin, B., J. S. Chon, J. A. MacGurn, F. Carlsson, T. L. Cheng, J. S. Cox, and E. J. Brown. 2007. A mycobacterium ESX-1-secreted virulence factor with unique requirements for export. PLoS Pathogens. 3(8):1051-1061.

Digiuseppe Champion, P. A., and J. S. Cox. 2007. Protein secretion systems in mycobacteria. Cellular Microbiology. 9(6):1376-1384.

DiGiuseppe Champion, P. A., S. A. Stanley, M. M. Champion, E. J. Brown, and J. S. Cox. 2006. C-terminal signal sequence promotes virulence factor secretion in mycobacterium tuberculosis. Science. 313(5793):1632-1636.

Brodin, P., L. Majlessi, L. Marsollier, M. I. De Jonge, D. Bottai, C. Demangel, J. Hinds, et al. 2006. Dissection of ESAT-6 system 1 of mycobacterium tuberculosis and impact on immunogenicity and virulence. Infection and Immunity. 74(1)88-98.

Fortune, S. M., A. Jaeger, D. A. Sarracino, M. R. Chase, C. M. Sassetti, D. R. Sherman, B. R. Bloom, and E. J. Rubin. 2005. Mutually dependent secretion of proteins required for mycobacterial virulence. Proceedings of the National Academy of Sciences of the United States of America. 102(30):10676-10681.

Maturation Pathway of Telomerase

An important step in the maturation pathway of telomerase has been discovered at the Stowers Institute’s Baumann Lab.  Telomerase uses part of an RNA subunit as a template to add telomeric DNA to the ends of chromosomes. The Baumann Lab found that this RNA subunit is first made as a longer inactive form that must be processed into a shorter mature form for telomerase to function.   The research is published in the Dec 5 issue of NATURE.

New Light Shed on Intracellular Molecular Motors

ScienceDaily (Dec. 15, 2008) — Biologists have known for decades that cells use tiny molecular motors to move chromosomes, mitochondria, and many other organelles within the cell, but no one has been able to understand what “steers” these engines to their destinations. Now, researchers at the University of Rochester have shed new light on how cells accomplish this feat, and the results may eventually lead to new approaches to fighting pathogens and neurological diseases.  The mechanisms that control the molecular motors are quite different from what biologists have previously believed.   Read the full story in the latest issue of Cell.

NYT List of “Notable” Books for 2008

The Book Review Section of the New York Times has released it’s annual list of books selected as the most notable books of the year.   There are 100 books on the list.  The ten ‘best’ books are:


DANGEROUS LAUGHTER:Thirteen Stories  By Steven Millhauser    Alfred A. Knopf

A MERCY  By Toni Morrison   Alfred A. Knopf

NETHERLAND   By Joseph O’Neill   Pantheon Books

2666   By Roberto Bolaño. Translated by Natasha Wimmer.  Farrar, Straus & Giroux

UNACCUSTOMED EARTH    By Jhumpa Lahiri  Alfred A. Knopf

Non Fiction

THE DARK SIDE: The Inside Story of How the War on Terror Turned Into a War on American Ideals
By Jane Mayer.  Doubleday

THE FOREVER WAR By Dexter Filkins.  Alfred A. Knopf

NOTHING TO BE FRIGHTENED OF  By Julian Barnes Alfred A. Knopf

THIS REPUBLIC OF SUFFERING: Death and the American Civil War  By Drew Gilpin Faust  Alfred A. Knopf

THE WORLD IS WHAT IT IS: The Authorized Biography of V. S. Naipaul  By Patrick French   Alfred A. Knopf

See the list of all 100 books chosen this year by the editors of the New York Times Book Review.

New Therapies Possible with “Regulatory T Cells”

ScienceDaily (Dec. 10, 2008) — A study published by Elsevier this month in Clinical Immunology describes a new method that facilitates the induction of a specific type of immune suppressive cells, called ‘regulatory T cells’ for therapeutic use. These immune suppressive cells show great potential for the treatment of autoimmune diseases and improving transplantation outcomes. Regulatory T cells are an important part of the immune system and can play a suppressive role, but naturally occur in low numbers.  Michael Albert and colleagues from the Ludwig-Maximilians-University in Munich, Germany, describe a unique strategy that facilitates the induction of regulatory T cells ex vivo with subsequent expansion to numbers adequate for immunotherapy.  Using an inexpensive, fast and simple high-yield method they generated regulatory T cells from small amounts of peripheral blood which, potentially, could be transferred back into a patient enabling a clinically desired immune suppression.   For a copy of this article please contact Markus Library: call Alisa at 8957, or email