Recommended Readings: Sebastian Klinge, Ph.D. Friday April 12, 2019

Friday Lectures

Friday, April 12, 2019  3:45 p.m

Caspary Auditorium

Sebastian Klinge Ph.D.

Assistant Professor and Head

Laboratory of Protein and Nucleic Acid Chemistry

The Rockefeller University

Nucleolar Assembly of the Small Ribosomal Subunit

 

Recommended Readings:

Empirical Articles

Chaker-Margot, Malik; Klinge, Sebastian. (2019). Assembly and early maturation of large subunit precursors. RNA. 25 (4): 465-471

Chaker-Margot, Malik; Barandun, Jonas; Hunziker, Mirjam; et al. (2017). Architecture of the yeast small subunit processome. SCIENCE. 355 (6321)

Barandun, Jonas; Chaker-Margot, Malik; Hunziker, Mirjam; et al. (2017). The complete structure of the small-subunit processome. NATURE STRUCTURAL AND MOLECULAR BIOLOGY. 24 (11): 944-+

Hunziker, Mirjam; Barandun, Jonas; Petfalski, Elisabeth; et al. (2016). UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly. NATURE COMMUNICATIONS. 7

Chaker-Margot, Malik; Hunziker, Mirjam; Barandun, Jonas; et al. (2015). Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis. NATURE STRUCTURAL AND MOLECULAR BIOLOGY. 22 (11): 920-923

Review Papers

Klinge, Sebastian; Woolford, John L., Jr. (2019). Ribosome assembly coming into focus. NATURE REVIEWS MOLECULAR CELL BIOLOGY. 20 (2): 116-131

Barandun, Jonas; Hunziker, Mirjam; Klinge, Sebastian. (2018). Assembly and structure of the SSU processome-a nucleolar precursor of the small ribosomal subunit. CURRENT OPINION IN STRUCTURAL BIOLOGY. 49: 85-93

Recommended Readings: Howard C. Hang, Ph.D. Monday February 25, 2019

Monday Lectures

Monday, February 25, 2019  4:00 p.m.

Carson Family Auditorium

Howard C. Hang Ph.D.

Richard E. Salomon Family Associate Professor and Head

Laboratory of Chemical Biology and Microbial Pathogenesis

The Rockefeller University

Microbiota Modulation of Host Immunity, the SagA continues

Recommended Readings:

Empirical Articles

Liu W, Zhou Y, Peng T, Zhou P, Ding X, Li Z, Zhong H, Xu Y, Chen S, Hang HC, Shao F. (2018). Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function. NATURE MICROBIOLOGY. 3 (9): 996-1009

Thinon, Emmanuelle; Fernandez, Joseph P.; Molina, Henrik; et al. (2018). Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites. JOURNAL OF PROTEOME RESEARCH. 17 (5): 1907-1922

Rangan, Kavita J.; Pedicord, Virginia A.; Wang, Yen-Chih; et al. (2016). A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens. SCIENCE. 353 (6306): 1434-1437

Pedicord, Virginia A.; Lockhart, Ainsley A. K.; Rangan, Kavita J.; et al. (2016). Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance. SCIENCE IMMUNOLOGY. 1 (3)    

Review Paper

Rangan, Kavita J.; Hang, Howard C. (2017). Biochemical Mechanisms of Pathogen Restriction by Intestinal Bacteria. TRENDS IN BIOCHEMICAL SCIENCES. 42 (11): 887-898

Westcott, Nathan P.; Hang, Howard C. (2014). Chemical reporters for exploring ADP-ribosylation and AMPylation at the host-pathogen interface.  CURRENT OPINION IN CHEMICAL BIOLOGY. 23: 56-62

Grammel, Markus; Hang, Howard C. (2013). Chemical reporters for biological discovery. NATURE CHEMICAL BIOLOGY. 9 (8): 475-484

Recommended Readings: David Julius, Ph.D. Friday February 8, 2019

Friday Lectures

Friday, February 8, 2019  3:45 p.m

Caspary Auditorium

David Julius Ph.D.

Morris Herzstein Chair in Molecular Biology and Medicine

Professor and Chair of Physiology

University of California

Natural Products as Probes of the Pain Pathway: From Physiology to Atomic Structure

Recommended Readings:

Empirical Articles

Gao, Yuan; Cao, Erhu; Julius, David; et al. (2016). TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action. NATURE. 534 (7607): 347-+

Paulsen, Candice E.; Armache, Jean-Paul; Gao, Yuan; et al. (2015). Structure of the TRPA1 ion channel suggests regulatory mechanisms. NATURE. 520 (7548): 511-+

Liao, Maofu; Cao, Erhu; Julius, David; et al. (2013). Structure of the TRPV1 ion channel determined by electron cryo-microscopy. NATURE. 504 (7478): 107-+

Cavanaugh, Daniel J.; Chesler, Alexander T.; Jackson, Alexander C.; et al. (2011). Trpv1 Reporter Mice Reveal Highly Restricted Brain Distribution and Functional Expression in Arteriolar Smooth Muscle Cells. JOURNAL OF NEUROSCIENCE. 31 (13): 5067-5077

Cordero-Morales, Julio F.; Gracheva, Elena O.; Julius, David. (2011). Cytoplasmic ankyrin repeats of transient receptor potential A1 (TRPA1) dictate sensitivity to thermal and chemical stimuli. PNAS. 108 (46): E1184-E1191

Cavanaugh, Daniel J.; Chesler, Alexander T.; Braz, Joao M.; et al. (2011). Restriction of Transient Receptor Potential Vanilloid-1 to the Peptidergic Subset of Primary Afferent Neurons Follows Its Developmental Downregulation in Nonpeptidergic Neurons. JOURNAL OF NEUROSCIENCE. 31 (28): 10119-10127

Review Papers

Bohlen, Christopher J.; Julius, David (2012). Receptor-targeting mechanisms of pain-causing toxins: How ow? TOXICON. 60 (3): 254-264

Basbaum, Allan I.; Bautista, Diana M.; Scherrer, Gregory; et al. (2009). Cellular and Molecular Mechanisms of Pain. CELL. 139 (2): 267-284

Book Chapter

Julius, David. (2013). TRP Channels and Pain. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY. 29: 355-384

 

Recommended Readings: Tom Muir, Ph.D. Friday January 25, 2019

Friday Lectures

Friday, January 25, 2019  3:45 p.m

Caspary Auditorium

Tom Muir Ph.D.

Professor of Chemistry and Chair

Department of Chemistry

Princeton University

Painting Chromatin with Synthetic Protein Chemistry

 

Recommended Readings:

Empirical Articles

Nacev, B.A., Feng, L., Bagert, J.D. Lemiesz, A., et al. (2019). The expanding landscape of ‘oncohistone’ mutations in human cancers. NATURE, in press

Wojcik, Felix; Dann, Geoffrey P.; Beh, Leslie Y.; et al. (2018). Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants. NATURE COMMUNICATIONS. 9

Lu, Chao; Jain, Siddhant U.; Hoelper, Dominik; et al. (2016). Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. SCIENCE. 352 (6287): 844-849

Nguyen, Uyen T. T.; Bittoval, Lenka; Mueller, Manuel M.; et al. (2014). Accelerated chromatin biochemistry using DNA-barcoded nucteosome libraries. NATURE METHODS. 11 (8): 834-840

Fierz, Beat; Kilic, Sinan; Hieb, Aaron R.; et al. (2012). Stability of Nucleosomes Containing Homogenously Ubiquitylated H2A and H2B Prepared Using Semisynthesis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134 (48): 19548-19551

Review Paper

David, Yael; Muir, Tom W. (2017). Emerging Chemistry Strategies for Engineering Native Chromatin. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 139 (27): 9090-9096

Mueller, Manuel M.; Muir, Tom W. (2015). Histones: At the Crossroads of Peptide and Protein Chemistry. CHEMICAL REVIEWS. 115 (6): 2296-2349

Chatterjee, Champak; Muir, Tom W. (2010). Chemical Approaches for Studying Histone Modifications. JOURNAL OF BIOLOGICAL CHEMISTRY. 285 (15): 11045-11050

Book Chapter

Holt, Matthew; Muir, Tom (2015). Application of the Protein Semisynthesis Strategy to the Generation of Modified Chromatin. ANNUAL REVIEW OF BIOCHEMISTRY. 84: 265-290

McGinty, Robert K.; Chatterjee, Champak; Muir, Tom W. (2009). Semisynthesis of Ubiquitylated Proteins. METHODS IN ENZYMOLOGY: NON-NATURAL AMINO ACIDS. 462: 225-243

Recommended Readings: David Baker, Ph.D. Friday January 11, 2019

Friday Lectures

Friday, January 11, 2018  3:45 p.m

Caspary Auditorium

David Baker Ph.D.

Professor of Biochemistry and Director of the Institute for Protein Design

University of Washington

The Coming of Age of De Novo Protein Design

 

Recommended Readings:

Science News

Scientists program proteins to pair exactly. December 19, 2018. Science Daily

Empirical Articles

Shen, Hao; Fallas, Jorge A.; Lynch, Eric; et al. (2018). De novo design of self-assembling helical protein filaments. SCIENCE. 362 (6415): 705-709

Marcos, Enrique; Chidyausiku, Tamuka M.; McShan, Andrew C.; et al. (2018). De novo design of a non-local beta-sheet protein with high stability and accuracy. NATURE STRUCTURAL AND MOLECULAR BIOLOGY. 25 (11): 1028-1030

Dou, Jiayi; Vorobieva, Anastassia A.; Sheffler, William; et al. (2018). De novo design of a fluorescence-activating beta-barrel. NATURE. 561 (7724): 485-+

Lu, Peilong; Min, Duyoung; DiMaio, Frank; et al. (2018). Accurate computational design of multipass transmembrane proteins. SCIENCE. 359 (6379): 1042-1046

Hosseinzadeh, Parisa; Bhardwaj, Gaurav; Mulligan, Vikram Khipple; et al. (2017). Comprehensive computational design of ordered peptide macrocycles. SCIENCE. 358 (6369): 1461-1466

Review Paper

Huang, Po-Ssu; Boyken, Scott E.; Baker, David. (2016). The coming of age of de novo protein design. NATURE. 537 (7620): 320-327

Fleishman, Sarel J.; Baker, David. (2012). Role of the Biomolecular Energy Gap in Protein Design, Structure, and Evolution. CELL. 149 (2): 262-273

Book Chapter

Thyme, Summer; Baker, David. (2014). Redesigning the Specificity of Protein-DNA Interactions with Rosetta. HOMING ENDONUCLEASES: METHODS AND PROTOCOLS. 1123: 265-282

Whitehead, Timothy A.; Baker, David; Fleishman, Sarel J. (2013). Computational Design of Novel Protein Binders and Experimental Affinity Maturation. METHODS IN PROTEIN DESIGN. 523: 1-19

 Leaver-Fay, Andrew; Tyka, Michael; Lewis, Steven M.; et al. (2011). Rosetta3: An Object-Oriented Software Suite for the Simulation and Design of Macromolecules. METHODS IN ENZYMOLOGY. 487: 545-574

 

Recommended Readings: Jue Chen, Ph.D., Monday December 10, 2018

Monday Lectures

Monday, December 10, 2018  4:00 p.m.

Carson Family Auditorium

Jue Chen, Ph.D.

William E. Ford Professor and Head

Laboratory of Membrane Biology and Biophysics

The Rockefeller University

CFTR, the Odd ABC Transporter Responsible for Cystic Fibrosis

 

Recommended Readings:

Empirical Articles

Kim, Youngjin; Chen, Jue. (2018). Molecular structure of human P-glycoprotein in the ATP-bound, outward-facing conformation. SCIENCE. 359 (6378): 915-919

Zhang, Zhe; Liu, Fangyu; Chen, Jue. (2017). Conformational Changes of CFTR upon Phosphorylation and ATP Binding. CELL. 170 (3): 483-+

Liu, Fangyu; Zhang, Zhe; Csanady, Laszlo; et al. (2017). Molecular Structure of the Human CFTR Ion Channel. CELL. 169 (1): 85-+

Zhang, Zhe; Chen, Jue. (2016). Atomic Structure of the Cystic Fibrosis Transmembrane Conductance Regulator. CELL. 167 (6): 1586-+

Oldham, Michael L.; Davidson, Amy L.; Chen, Jue. (2008). Structural insights into ABC transporter mechanism. CURRENT OPINION IN STRUCTURAL BIOLOGY. 18 (6): 726-733

Review Paper

Davidson, Amy L.; Dassa, Elie; Orelle, Cedric; et al. (2008). Structure, function, and evolution of bacterial ATP-binding cassette systems. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 72 (2): 317-364

 

Recommended Readings: Howard C. Hang, Ph.D. Friday October 19, 2018

Friday Lectures

Friday, October 19, 2018  3:45 p.m.

Caspary Auditorium

Howard C. Hang Ph.D.

Richard E. Salomon Family Associate Professor and Head

Laboratory of Chemical Biology and Microbial Pathogenesis

The Rockefeller University

Chemical Dissection of Host-Microbe Interactions

Recommended Readings:

Empirical Articles

Liu W, Zhou Y, Peng T, Zhou P, Ding X, Li Z, Zhong H, Xu Y, Chen S, Hang HC, Shao F. (2018). Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function. NATURE MICROBIOLOGY. 3 (9): 996-1009

Thinon, Emmanuelle; Fernandez, Joseph P.; Molina, Henrik; et al. (2018). Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites. JOURNAL OF PROTEOME RESEARCH. 17 (5): 1907-1922

Westcott N, Fernandez JP, Molina H, Hang HC. (2017). Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. NATURE CHEMICAL BIOLOGY. 13 (3): 302-308

Peng T, Hang HC. (2016). Site-Specific Bioorthogonal Labeling for Fluorescence Imaging of Intracellular Proteins in Living Cells. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 138 (43): 14423-14433

Rangan, Kavita J.; Pedicord, Virginia A.; Wang, Yen-Chih; et al. (2016). A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens. SCIENCE. 353 (6306): 1434-1437

Percher, Avital; Ramakrishnan, Srinivasan; Thinon, Emmanuelle; et al. (2016). Mass-tag labeling reveals site-specific and endogenous levels of protein S-fatty acylation. PNAS. 113 (16): 4302-7

Review Paper

Peng, Tao; Thinon, Emmanuelle; Hang, Howard C. (2016). Proteomic analysis of fatty-acylated proteins. CURRENT OPINION IN CHEMICAL BIOLOGY. 30: 77-86

Westcott, Nathan P.; Hang, Howard C. (2014). Chemical reporters for exploring ADP-ribosylation and AMPylation at the host-pathogen interface.  CURRENT OPINION IN CHEMICAL BIOLOGY. 23: 56-62

Grammel, Markus; Hang, Howard C. (2013). Chemical reporters for biological discovery. NATURE CHEMICAL BIOLOGY. 9 (8): 475-484

Recommended Readings: David Pellman, M.D. Friday June 1, 2018

Friday Lectures

Friday, June 1, 2018  3:45 p.m.

Caspary Auditorium

David Pellman M.D.

Margaret M. Dyson Professor

Department of Cell Biology

Harvard Medical School

Mechanisms of Rapid Genome Evolution

Recommended Readings:

Science News

Dana-Farber Cancer Institute. Researchers identify origin of chromosomal oddity in some cancer cells. May 27, 2015. EurekAlert

Empirical Articles

Marteil, Gaelle; Guerrero, Adan; Vieira, Andre F.; et al. (2018). Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation. NATURE COMMUNICATIONS. 9    

Zhang, Cheng-Zhong; Spektor, Alexander; Cornils, Hauke; et al. (2015). Chromothripsis from DNA damage in micronuclei. NATURE. 522 (7555): 179-+

Selmecki, Anna M.; Maruvka, Yosef E.; Richmond, Phillip A.; et al. (2015). Polyploidy can drive rapid adaptation in yeast. NATURE. 519 (7543): 349-+

Crasta, Karen; Ganem, Neil J.; Dagher, Regina; et al. (2012). DNA breaks and chromosome pulverization from errors in mitosis. NATURE. 482 (7383): 53-U70

Carter, Scott L.; Cibulskis, Kristian; Helman, Elena; et al. (2012). Absolute quantification of somatic DNA alterations in human cancer. NATURE BIOTECHNOLOGY. 30 (5): 413-+

Ganem, Neil J.; Godinho, Susana A.; Pellman, David. (2009). A mechanism linking extra centrosomes to chromosomal instability. NATURE. 460 (7252): 278-U146

Review Paper

Zhang, Cheng-Zhong; Leibowitz, Mitchell L.; Pellman, David. (2013). Chromothripsis and beyond: rapid genome evolution from complex chromosomal rearrangements. GENES AND DEVELOPMENT. 27 (23): 2513-2530   

Gordon, David J.; Resio, Benjamin; Pellman, David. (2012). Causes and consequences of aneuploidy in cancer. NATURE REVIEWS GENETICS. 13 (3): 189-203

Book Chapter

Leibowitz, Mitchell L.; Zhang, Cheng-Zhong; Pellman, David. (2015). Chromothripsis: A New Mechanism for Rapid Karyotype Evolution. ANNUAL REVIEW OF GENETICS. 49: 183-211

Recommended Readings: Stephen Elledge, Ph.D. Friday May 18, 2018

Friday Lectures

Friday, May 18, 2018  3:45 p.m.

Caspary Auditorium

Stephen J. Elledge Ph.D.

Gregor Mendel Professor

Department of Genetics

Harvard Medical School

Microgenetics: What We Can Learn About Protein Degradation from Bits of Genes

Recommended Readings:

Science News

Your viral infection history in a single drop of blood. June 4, 2015. ScienceDaily

Empirical Articles

Sack, Laura Magill; Davoli, Teresa; Li, Mamie Z.; et al. (2018). Profound Tissue Specificity in Proliferation Control Underlies Cancer Drivers and Aneuploidy Patterns. CELL. 173 (2): 499-+

Liao, Sida; Davoli, Teresa; Leng, Yumei; et al. (2017). A genetic interaction analysis identifies cancer drivers that modify EGFR dependency. GENES AND DEVELOPMENT. 31 (2): 184-196

Xu, George J.; Kula, Tomasz; Xu, Qikai; et al. (2015). Comprehensive serological profiling of human populations using a synthetic human virome. SCIENCE. 348 (6239)

Emanuele, Michael J.; Elia, Andrew E. H.; Xu, Qikai; et al. (2011). Global Identification of Modular Cullin-RING Ligase Substrates. CELL. 147 (2): 459-474

Larman, H. Benjamin; Zhao, Zhenming; Laserson, Uri; et al. (2011). Autoantigen discovery with a synthetic human peptidome. NATURE BIOTECHNOLOGY. 29 (6): 535-U101

Yen, Hsueh-Chi Sherry; Xu, Qikai; Chou, Danny M.; et al. (2008). Global Protein Stability Profiling in Mammalian Cells. SCIENCE. 322 (5903): 918-923

Yen, Hsueh-Chi Sherry; Elledge, Stephen J. (2008). Identification of SCF Ubiquitin Ligase Substrates by Global Protein Stability Profiling. SCIENCE. 322 (5903): 923-929

Review Paper

Ciccia, Alberto; Elledge, Stephen J. (2010). The DNA Damage Response: Making It Safe to Play with Knives. MOLECULAR CELL. 40 (2): 179-204

Luo, Ji; Solimini, Nicole L.; Elledge, Stephen J. (2009). Principles of Cancer Therapy: Oncogene and Non-oncogene Addiction. CELL. 136 (5): 823-837

 

Recommended Readings: Stephen Goff, Ph.D. Friday May 11, 2018

Friday Lectures

Friday, May 11, 2018  3:45 p.m.

Caspary Auditorium

Stephen Goff Ph.D.

Higgins Professor

Department of Biochemistry and Molecular Biophysics

Columbia University College of Physicians and Surgeons

Quiet, Please: Silencing Retroviral DNAs

Recommended Readings:

Empirical Articles

Lee, Andreia; CingOz, Oya; Sabo, Yosef; et al. (2018). Characterization of interaction between Trim28 and YY1 in silencing proviral DNA of Moloney murine leukemia virus. VIROLOGY. 516: 165-175

Geis, Franziska K.; Galla, Melanie; Hoffmann, Dirk; et al. (2017). Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells. RETROVIROLOGY. 14

Wang, Gary Z.; Goff, Stephen P. (2017). Transcriptional Silencing of Moloney Murine Leukemia Virus in Human Embryonic Carcinoma Cells. JOURNAL OF VIROLOGY. 91 (1)

Wang, Cheng; Goff, Stephen P. (2017). Differential control of retrovirus silencing in embryonic cells by proteasomal regulation of the ZFP809 retroviral repressor. PNAS. 114 (6): E922-E930

Wang, Gary Z.; Wang, Ying; Goff, Stephen P. (2016). Histones Are Rapidly Loaded onto Unintegrated Retroviral DNAs Soon after Nuclear Entry. CELL HOST MICROBE.  20 (6): 798-809

Griffin, Daniel O.; Goff, Stephen P. (2015). HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34(+) Cells. JOURNAL OF VIROLOGY. 89 (15): 8096-8100

Yang, Bin Xia; El Farran, Chadi A.; Guo, Hong Chao; et al. (2015). Systematic Identification of Factors for Provirus Silencing in Embryonic Stem Cells. CELL. 163 (1): 230-245

Review Paper

Schlesinger, Sharon; Goff, Stephen P. (2015). Retroviral Transcriptional Regulation and Embryonic Stem Cells: War and Peace. MOLECULAR AND CELLULAR BIOLOGY. 35 (5): 770-777

Studamire, Barbara; Goff, Stephen P. (2010). Interactions of Host Proteins with the Murine Leukemia Virus Integrase. VIRUSES. 2 (5): 1110-1145