Recommended Readings: Joseph Schlessinger, Ph.D., May 13

Friday Lecture Series
Friday, May 13, 2016
3:45 p.m., Caspary Auditorium

Joseph Schlessinger, Ph.D.
William H. Prusoff Professor and Chair,
Department of Pharmacology,
Yale School of Medicine

Cell Signaling by Receptor Tyrosine Kinases: From Basic Principles to Cancer Therapy

Recommended Readings:

Empirical Articles

Bae, J. H., Lew, E. D., Yuzawa, S., Tomé, F., Lax, I., & Schlessinger, J. (2009). The selectivity of receptor tyrosine kinase signaling is controlled by a secondary SH2 domain binding site. Cell, 138(3), 514-524. doi: 10.1016/j.cell.2009.05.028.

Chung, I., Akita, R., Vandlen, R., Toomre, D., Schlessinger, J., & Mellman, I. (2010). Spatial control of EGF receptor activation by reversible dimerization on living cells. Nature, 464(7289), 783-787. doi: 10.1038/nature08827.

Yuzawa, S., Opatowsky, Y., Zhang, Z., Mandiyan, V., Lax, I., & Schlessinger, J. (2007). Structural basis for activation of the receptor tyrosine kinase KIT by stem cell factor. Cell, 130(2), 323-334.

Review Papers

Lemmon, M. A., & Schlessinger, J. (2010). Cell signaling by receptor tyrosine kinases. Cell, 141(7), 1117-1134. doi: 10.1016/j.cell.2010.06.011.

Schlessinger, J. (2014). Receptor tyrosine kinases: legacy of the first two decades. Cold Spring Harbor Perspectives in Biology, 6(3), a008912. doi: 10.1101/cshperspect.a008912.

Recommended Readings: Andrew Kruse Ph.D. March 3

Monday, March 3, 2014
4:00 p.m., Carson Family Auditorium

Andrew Kruse, Ph.D.
Department of Molecular and Cellular Physiology
Stanford University School of Medicine

Structural insights into G protein-coupled receptor activation and allosteric modulation

Recommended Readings:

Empirical Papers

Haga, K., Kruse, A. C., Asada, H., Yurugi-Kobayashi, T., Shiroishi, M., Zhang, C., … Kobayashi, T. (2012). Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist. Nature, 482(7386), 547–551. doi:10.1038/nature10753

Kruse, A. C., Hu, J., Pan, A. C., Arlow, D. H., Rosenbaum, D. M., Rosemond, E., … Kobilka, B. K. (2012). Structure and dynamics of the M3 muscarinic acetylcholine receptor. Nature, 482(7386), 552–556. doi:10.1038/nature10867

Kruse, A. C., Ring, A. M., Manglik, A., Hu, J., Hu, K., Eitel, K., … Kobilka, B. K. (2013). Activation and allosteric modulation of a muscarinic acetylcholine receptor. Nature, 504(7478), 101–106. doi:10.1038/nature12735

Kruse, A. C., Weiss, D. R., Rossi, M., Hu, J., Hu, K., Eitel, K., … Shoichet, B. K. (2013). Muscarinic receptors as model targets and antitargets for structure-based ligand discovery. Molecular Pharmacology, 84(4), 528–540. doi:10.1124/mol.113.087551

Ring, A. M., Manglik, A., Kruse, A. C., Enos, M. D., Weis, W. I., Garcia, K. C., & Kobilka, B. K. (2013). Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody. Nature, 502(7472), 575–579. doi:10.1038/nature12572

Review Articles

Kruse, A. C., Li, J., Hu, J., Kobilka, B. K., & Wess, J. (2013). Novel insights into M3 muscarinic acetylcholine receptor physiology and structure. Journal of Molecular Neuroscience. doi:10.1007/s12031-013-0127-0

Kruse, A. C., Manglik, A., Kobilka, B. K., & Weis, W. I. (2013). Applications of molecular replacement to G protein-coupled receptors. Acta Crystallographica. Section D, Biological Crystallography, 69(11), 2287–2292. doi:10.1107/S090744491301322X

Delivering Paricles Directly Into Cells

Scientists who developed a technology for identifying and targeting unique protein receptor ZIP Codes on the cellular surface have found a way to penetrate the outer membrane and deliver engineered particles — called iPhage — to organelles inside the cell. 

In a paper recently published online in Nature Communications, the team led by researchers at The University of Texas MD Anderson Cancer Center reports packaging the phage particles with a peptide called penetratin to reach inside the cell.

How Cells Detect Tissue Damage And Proceed With Repairs

A  Wellcome Trust-funded study, led by biochemists at the University of Bristol, examined the signalling process in damaged tissue cells and identified the cellular mechanisms responsible for activating effective repair. 

In healthy adults the majority of tissue cells lie dormant unless challenged by wounding, at which point they sense a change in the molecular environment. Plasma leaking from damaged blood vessels and causes fibroblast cells to migrate into the damaged tissue, contract the wound, and plug the gap by depositing a substance such as collagen, which provides the structural support.   Read more about this research in Developmental Cell.

Scripps Research Team Discovers New Details About Medically Important Protein Family

Scientists from The Scripps Research Institute have determined a new structure from a medically important superfamily of proteins. The structure should help instruct the design of a new kind of therapeutics for conditions ranging from Parkinson’s disease to inflammation.   The study, published on March 10, 2011, in Science Express provides important insights into how this large family of proteins, called G protein-coupled receptors (GPCRs), can recognize and respond to a wide array of signals, including odors, hormones, neurotransmitters, and light.

Recommended Readings: Sarah Schlesinger M.D. March 7, 2011

Monday Lecture Series

Vaccines that Target Dendritic Cells

Sarah Schlesinger  M.D.

Senior Attending Physician and Associate Professor of Clinical Investigation

 Cellular Physiology and Immunology

March 7, 2011

4:00 p.m.-5:00 p.m. Caspary Auditorium

Recommended Readings

Dhodapkar MV; Sznol M; Wang D, et al.  2010.  Early development of CDX-1401, a novel vaccine targeting NY-ESO-1 to the dendritic cel receptor DEC-205.  Journal of Immunotherapy.  33(8):895-896   Request Article from Markus Library. 

Wanialla CN; Faul EJ; Gomme EA, et al.  2010.  Dendritic cells infected by recombinant rabies virus vaccine vector expressing HIV-1 Gag are immunogenic even in the presence of vector-specific  immunityVaccine.  29(1):130-140

Fiorentini S; Giagulli C; Caccuri F, et al.  2010.  HIV-1 matrix protein p17: a candidate antigen for therapeutic vaccines against AIDS.   Pharmacology & Therapeutics.  128(3):433-444  Request Article from Markus Library.

De Groot A; Buhlmann J; Weber C, et al.  2010.  De-Tolerization of anti-DEC-205 for HIV vaccine delivery.  (abstract only)  AIDS Research and Human Retroviruses.   26(10):A135-A136

Ahlers, JD; and  IM Belyakov.  2009.  Strategies for optimizing targeting and delivery of mucosal HIV vaccinesEuropean Journal of Immunology.  39(10):2657-2669

Kloverpris HN; Karlsson I; Thorn M, et al.  2009.   Immune hierarchy among HIV-1 CD8+T cell epitopes delivered by dendritic cells depends on MHC-I binding irrespective of mode of loading and immunization of HLA-A*0201 mice.  APMIS  117(11):8489-855

Demberg T; and M. Robert-Guroff.  2009.  Mucosal immunity and protection against HIV-SIV infection: strategies and challenges for vaccine design.  International Reviews of Immunology.  28(1-2):20-28   Please request from Markus Library.

Gruber A; Chalmers AS; Rasmussem RA, et al.  2007.  Dendritic cell-based vaccine strategy against human immunodeficiency virus clade C: skewing the immune response toward a helper T cell type 2 profile.   Viral Immunology.  20(1):160-169.

Recommended Readings: Mary Jeanne Kreek, MD Nov 8 2010

Monday Lecture Series

Translational Research:

Contributions of Gene Variants, Endorphins, and Stress Responsivity to Specific Addictions

Mary Jeanne Kreek, MD

Laboratory of the Biology of Addictive Diseases

The Rockefeller University

November 8, 2010

4:15 p.m.  Caspary Hall

Review Article:

Schwarzer C.   2009.  30 years of dynorphins – New insights on their functions in neuropsychiatric diseases.  PHARMACOLOGY & THERAPEUTICS 123(3):353-370 Please request from Markus Library.

Recommended Articles:

Shin AC, Pistell PJ, Phifer CB, et al.  2010.  Reversible suppression of food reward behavior buy chronic mu-opioid receptor antagonism in the nucleus accumbens. NEUROSCIENCE 170(2): 580-588

Mizoguchi H, Watanabe C, Osada S, et al.   2010.  Lack of a rewarding effect and a locomotor-enhancing effect of the selective mu-opioid receptor agonist amidino-TAPA.  PSYCHOPHARMACOLOGY 212(2): 215-225

Mague SD, Blendy JA.  2010.  OPRM1 SNP (A118G): Involvement in disease development, treatment response, and animal models. DRUG AND ALCOHOL DEPENDENCE 108(3, Special Issue SI): 172-182  Request from Markus Library.

Kreek MJ.  2010.  Overview and historical perspective of four papers presented on research related to the endogenous opioid system.  DRUG AND ALCOHOL DEPENDENCE 10(3,  Special Issue SI): 195-199  Request from Markus Library.

Bruchas MR, Land BB, Chavkin C.  2010.  The dynorphin/kappa opioid system as a modulator of stress-induced and pro-addictive behaviors. BRAIN RESEARCH 1314( Special Issue SI): 44-55

Zhou Y, Proudnikov D, Yuferov V, et al.   2010.  Drug-induced and genetic alterations in stress-responsive systems: Implications for specific addictive diseases. BRAIN RESEARCH 1314(Special Issue SI): 235-252

Kreek MJ, Zhou Y, Butelman ER, et al.  2009.  Opiate and cocaine addiction: from bench to clinic and back to the bench. CURRENT OPINION IN PHARMACOLOGY 9(1): 74-80

Vitamin D Receptor Binding Sites Identified

NEW YORK (GenomeWeb News) – Vitamin D seems to influence the activity of scores of genes across the genome and may influence some genes implicated in some human diseases, including some autoimmune conditions and cancers, according to a new study in which researchers mapped vitamin D receptor binding sites across the genome and gauged vitamin D-related expression patterns.

An international research team led by investigators at the Wellcome Trust Centre for Human Genetics used chromatin immunoprecipitation coupled with massively parallel sequencing to find nearly 3,000 vitamin D receptor binding sites in cells treated with active vitamin D. Many of these sites seemed to cluster near genes linked to human diseases and other traits through past genome-wide association studies, they noted.  The study appears in Genome Research.

Recommended Readings: Leslie Vosshall Ph.D. Nov 30, 2009

Monday Lecture


Olfaction: From Perception to Receptors

Leslie Vosshall PhD

Chemers Family Associate Professor; Investigator HHMI
Laboratory of Neurogenetics and Behavior

The Rockefeller University

Nov. 30, 2009

Welch Hall, Level Two   4 p.m.

Recommended Articles:

 Su CY, Menuz K, Carlson JR.   2009.  Olfactory Perception: Receptors, Cells, and Circuits.  CELL  139(1): 45-59

  Wilson RI .  2008. Neural and behavioral mechanisms of olfactory perception. CURRENT OPINION IN NEUROBIOLOGY  18(4): 408-412

 Gottfried JA.  2008.  Perceptual and Neural Plasticity of Odor Quality Coding in the Human Brain.  CHEMOSENSORY PERCEPTION   1(2): 127-135    Request article from Markus Library.

Benton R, Sachse S, Michnick S, Vosshall L.  2006  Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo.  PLoS Biology  4(2):240-257   

 Gottfried JA, Dolan RJ. 2003.  The nose smells what the eye sees: Crossmodal visual facilitation of human olfactory perceptionNEURON   39(2): 375-386

Ha TS, Smith DP.  2009.  Odorant and pheromone receptors in insects. Frontiers in Cellular Neuroscience.  3:10.

Menashe I, Abaffy T, Hasin Y, Goshen S, Yahalom V, Luetje CW, Lancet D.  2007.  Genetic elucidation of human hyperosmia to isovaleric acid. PLoS Biology  5(11):e284.

Yannis Michalakis & François Renaud.  2009.  Malaria: Evolution in vector control. Nature 462, 298-300

Understanding metastasis: Collective Cell Migration Controlled by Wnt and Fgf Signaling

In the November 11, 2008 issue of Developmental Cell, investigators from the University of Utah School of Medicine report the results of their studies in an article entitled Wnt/B-Catenin and Fgf Signaling Control Collective Cell Migration by Restricting Chemokine Receptor Expression.  These studies demonstrate a link between Wnt and Fgf signaling pathways in zebrafish and their impact on collective cell migration. 

The Wnt pathway regulates cell-to-cell communication in embryogenesis and cancer and Fgf influences embryongenesis, healing, and cell proliferation.  Piotrowski and Aman demonstrate for the first time that the interaction between Wnt and Fgf pathways is critical for collective cell migration.  Each pathway can restrict chemokine receptor expression and thereby elucidate how some types of cancer metastisize. 

Extracted from Developmental Cell and ScienceDaily.