Recommended Readings: Liangcai Gu, Ph.D. January 7

Special Lecture
Wednesday, January 7, 2015
4:00 p.m., Carson Family Auditorium (CRC)

Liangcai Gu, Ph.D.
Research Fellow,
Department of Genetics,
Harvard Medical School

In Situ Sequencing of Molecular Interactomes

Recommended Readings

Empirical Articles

Gu, L., Li, C., Aach, J., Hill, D. E., Vidal, M., & Church, G. M. (2014). Multiplex single-molecule interaction profiling of DNA-barcoded proteins. Nature, 515(7528), 554–557. doi:10.1038/nature13761

Gu, L., Wang, B., Kulkarni, A., Geders, T. W., Grindberg, R. V, Gerwick, L., … Sherman, D. H. (2009). Metamorphic enzyme assembly in polyketide diversification. Nature, 459(7247), 731–735. doi:10.1038/nature07870

Li, J., Gu, L., Aach, J., & Church, G. M. (2014). Improved cell-free RNA and protein synthesis system. PLOS ONE, 9(9), e106232. doi:10.1371/journal.pone.0106232

Review Papers

Jones, A. C., Gu, L., Sorrels, C. M., Sherman, D. H., & Gerwick, W. H. (2009). New tricks from ancient algae: natural products biosynthesis in marine cyanobacteria. Current Opinion in Chemical Biology, 13(2), 216–23. doi:10.1016/j.cbpa.2009.02.019

Recommended Readings: Louis M. Staudt, M.D., Ph.D. January 9

Friday Lecture Series
Friday, January 9, 2015
3:45 p.m., Caspary Auditorium

Louis M. Staudt, M.D., Ph.D.
Director, Center for Cancer Genomics
Co-chief, Lymphoid Malignancies Branch
Head, Molecular Biology of Lymphoid Malignancies Section,
Center for Cancer Research,
National Cancer Institute at the National Institutes of Health

Therapy of Lymphoma Inspired by Functional and Structural Genomics

Recommended Readings

Empirical Articles

Alizadeh, A., Eisen, M., Davis, R., & Ma, C. (2000). Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature, 403(6769), 503-511.

Ngo, V. N., Davis, R. E., Lamy, L., Yu, X., Zhao, H., Lenz, G., … Staudt, L. M. (2006). A loss-of-function RNA interference screen for molecular targets in cancer. Nature, 441(7089), 106–110. doi:10.1038/nature04687

Rosenwald, A., Wright, G., Chan, W. C., Connors, J. M., Campo, E., Fisher, R. I., … Staudt, L. M. (2002). The use of molecular profiling to predict survival after chemotherapy for diffuse large-B-cell lymphoma. The New England Journal of Medicine, 346(25), 1937–1947. doi:10.1056/NEJMoa012914

Yang, Y., III, A. S., Emre, N., & Ceribelli, M. (2012). Exploiting synthetic lethality for the therapy of ABC diffuse large B cell lymphoma. Cancer Cell, 21(6), 723–737. doi:10.1016/j.ccr.2012.05.024.Exploiting

Review Papers

Lenz, G., & Staudt, L. M. (2010). Aggressive lymphomas. The New England Journal of Medicine, 362(15), 1417–1429. doi:10.1056/NEJMra0807082

Shaffer, A. L., Young, R. M., & Staudt, L. M. (2012). Pathogenesis of human B cell lymphomas. Annual Review of Immunology, 30, 565–610. doi:10.1146/annurev-immunol-020711-075027

Recommended Readings: Stephen Quake, Ph.D. November 12

Special Seminar Series
Wednesday, November 12, 2014,
4:00 p.m., Carson Family Auditorium (CRC)

Stephen Quake, Ph.D.
Lee Otterson Professor,
Bioengineering and Applied Physics,
Stanford University
Investigator,
Howard Hughes Medical Institute

Single Cell Genomics

Recommended Readings

Empirical Papers

Ashley, E. a, Butte, A. J., Wheeler, M. T., Chen, R., Klein, T. E., Dewey, F. E., … Altman, R. B. (2010). Clinical assessment incorporating a personal genome. Lancet, 375(9725), 1525–1535. doi:10.1016/S0140-6736(10)60452-7

Fan, H. C., Gu, W., Wang, J., Blumenfeld, Y. J., El-Sayed, Y. Y., & Quake, S. R. (2012). Non-invasive prenatal measurement of the fetal genome. Nature, 487(7407), 320–324. doi:10.1038/nature11251

Pushkarev, D., Neff, N. F., & Quake, S. R. (2009). Single-molecule sequencing of an individual human genome. Nature Biotechnology, 27(9), 847–850. doi:10.1038/nbt.1561

Wang, J., Fan, H. C., Behr, B., & Quake, S. R. (2012). Genome-wide single-cell analysis of recombination activity and de novo mutation rates in human sperm. Cell, 150(2), 402–412. doi:10.1016/j.cell.2012.06.030

Review Papers

Kalisky, T., Blainey, P., & Quake, S. R. (2011). Genomic analysis at the single-cell level. Annual Review of Genetics, 45, 431–445. doi:10.1146/annurev-genet-102209-163607

Melin, J., & Quake, S. R. (2007). Microfluidic large-scale integration: the evolution of design rules for biological automation. Annual Review of Biophysics and Biomolecular Structure, 36, 213–231. doi:10.1146/annurev.biophys.36.040306.132646

Recommended Readings: David Kingsley, Ph.D.

Friday Lecture Series

The Fairfield Osborn Memorial Lecture

Fishing for the Secrets of Vertebrate Evolution

David Kingsley, Ph.D., professor, developmental biology, Stanford University;

investigator, Howard Hughes Medical Institute

April 18, 2014

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Readings

han, Y. F., Marks, M. E., Jones, F. C., Villarreal Jr., G., Shapiro, M. D., Brady, S. D., . . . Kingsley, D. M. (2010). Adaptive evolution of pelvic reduction in sticklebacks by recurrent deletion of a pitxl enhancer. Science, 327(5963), 302-305

Haussler, D., O’Brien, S. J., Ryder, O. A., Keith Barker, F., Clamp, M., Crawford, A. J., . . . Turner, S. (2009). Genome 10K: A proposal to obtain whole-genome sequence for 10000 vertebrate species. Journal of Heredity, 100(6), 659-674

Jones, F. C., Grabherr, M. G., Chan, Y. F., Russell, P., Mauceli, E., Johnson, J., . . . Kingsley, D. M. (2012). The genomic basis of adaptive evolution in threespine sticklebacks. Nature, 484(7392), 55-61

Kingsley, D. M., Zhu, B., Osoegawa, K., De Jong, P. J., Schein, J., Marra, M., . . . Myers, R. (2004). New genomic tools for molecular studies of evolutionary change in threespine sticklebacks. Behaviour, 141(11-12), 1331-1344

Lowe, C. B., Kellis, M., Siepel, A., Raney, B. J., Clamp, M., Salama, S. R., . . . Haussler, D. (2011). Three periods of regulatory innovation during vertebrate evolution. Science, 333(6045), 1019-1024

Shapiro, M. D., Bell, M. A., & Kingsley, D. M. (2006). Parallel genetic origins of pelvic reduction in vertebrates. Proceedings of the National Academy of Sciences of the United States of America, 103(37), 13753-13758

 

Recommended Readings: Titia de Lange, Ph.D.

Friday Lecture Series

Breaking Bad: How Telomeres Hide from the DNA Damage Response

Titia de Lange, Ph.D., Leon Hess Professor, Head of the Laboratory of Cell Biology and Genetics, The Rockefeller University

February 28, 2014

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Readings

Davoli, T., Denchi, E. L., & de Lange, T. (2010). Persistent telomere damage induces bypass of mitosis and tetraploidy. Cell, 141(1), 81-93

Hockemeyer, D., Sfeir, A. J., Shay, J. W., Wright, W. E., & De Lange, T. (2005). POT1 protects telomeres from a transient DNA damage response and determines how human chromosomes end. EMBO Journal, 24(14), 2667-2678

Jacobs, J. J. L., & De Lange, T. (2005). p16INK4a as a second effector of the telomere damage pathway. Cell Cycle, 4(10), 1364-1368

Konishi, A., & De Lange, T. (2008). Cell cycle control of telomere protection and NHEJ revealed by a ts mutation in the DNA-binding domain of TRF2. Genes and Development, 22(9), 1221-1230

Sfeir, A., & De Lange, T. (2012). Removal of shelterin reveals the telomere end-protection problem. Science, 336(6081), 593-597

Sfeir, A., Kabir, S., Van Overbeek, M., Celli, G. B., & De Lange, T. (2010). Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Science, 327(5973), 1657-1661

Wu, P., Takai, H., & De Lange, T. (2012). Telomeric 3′ overhangs derive from resection by Exo1 and apollo and fill-in by POT1b-associated CST. Cell, 150(1), 39-52

 

Recommended Readings: Michael Strand, Ph.D.

Friday Lecture Series

Polydnaviruses: Viral Mutualists and Nature’s Genetic Engineers

Michael Strand, Ph.D., Regents Professor, department of entomology,

University of Georgia

February 21, 2014

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Readings

Beck, M. H., & Strand, M. R. (2007). A novel polydnavirus protein inhibits the insect prophenoloxidase activation pathway. Proceedings of the National Academy of Sciences of the United States of America, 104(49), 19267-19272

Burke, G. R., Thomas, S. A., Eum, J. H., & Strand, M. R. (2013). Mutualistic polydnaviruses share essential replication gene functions with pathogenic ancestors. PLoS Pathogens, 9(5)

Strand, M. R., & Burke, G. R. (2012). Polydnaviruses as symbionts and gene delivery systems. PLoS Pathogens, 8(7), 5

Thoetkiattikul, H., Beck, M. H., & Strand, M. R. (2005). Inhibitor κB-like proteins from a polydnavirus inhibit NF-κB activation and suppress the insect immune response. Proceedings of the National Academy of Sciences of the United States of America, 102(32), 11426-11431

Webb, B. A., Strand, M. R., Dickey, S. E., Beck, M. H., Hilgarth, R. S., Barney, W. E., . . . Witherell, R. A. (2006). Polydnavirus genomes reflect their dual roles as mutualists and pathogens. Virology, 347(1), 160-174

 

Recommended Readings: Poornima Parameswaran Ph.D. February 10

SPECIAL SEMINAR
Monday, February 10, 2014
4:00 p.m., Carson Family Auditorium

Poornima Parameswaran, Ph.D.

Postdoctoral Research Fellow
School of Public Health
University of California, Berkeley

Virome & Immunome Diversity in Dengue & other Infectious Diseases

Recommended Readings:

Empirical Papers

Parameswaran, P., Charlebois, P., Tellez, Y., Nunez, A., Ryan, E. M., Malboeuf, C. M., … Henn, M. R. (2012). Genome-wide patterns of intrahuman dengue virus diversity reveal associations with viral phylogenetic clade and interhost diversity. Journal of Virology, 86(16), 8546–8558. doi:10.1128/JVI.00736-12

Parameswaran, P., Liu, Y., Roskin, K. M., Jackson, K. K. L., Dixit, V. P., Lee, J.-Y., … Fire, A. Z. (2013). Convergent antibody signatures in human dengue. Cell Host & Microbe, 13(6), 691–700. doi:10.1016/j.chom.2013.05.008

Parameswaran, P., Sklan, E., Wilkins, C., Burgon, T., Samuel, M. a, Lu, R., … Fire, A. Z. (2010). Six RNA viruses and forty-one hosts: viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems. PLoS pathogens, 6(2), e1000764. doi:10.1371/journal.ppat.1000764

Review Articles

Fontana, J. M., Alexander, E., & Salvatore, M. (2012). Translational research in infectious disease: current paradigms and challenges ahead. Translational Research, 159(6), 430–453. doi:10.1016/j.trsl.2011.12.009

Wylie, K. M., Weinstock, G. M., & Storch, G. a. (2012). Emerging view of the human virome. Translational Research, 160(4), 283–290. doi:10.1016/j.trsl.2012.03.006

Recommended Readings: Martin Kampmann Ph.D. Monday, January 27

SPECIAL SEMINAR
Monday, January 27, 2014
4:00 p.m., Carson Family Auditorium

Martin Kampmann, Ph.D.
Postdoctoral Scholar
University of San Francisco

Toward a Systematic Understanding of the Mammalian Proteostasis Network and its Therapeutic Potential

Recommended Readings:

Empirical Papers

Bassik, M. C., Kampmann, M., Lebbink, R. J., Wang, S., Hein, M. Y., Poser, I., … Weissman, J. S. (2013). A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. Cell, 152(4), 909–922. doi:10.1016/j.cell.2013.01.030

Kampmann, M., Bassik, M. C., & Weissman, J. S. (2013). Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America, 110(25), E2317–2326. doi:10.1073/pnas.1307002110

Review Articles

Balch, W. E., Morimoto, R. I., Dillin, A., & Kelly, J. W. (2008). Adapting proteostasis for disease intervention. Science, 319(5865), 916–919. doi:10.1126/science.1141448

Hartl, F. U., Bracher, A., & Hayer-Hartl, M. (2011). Molecular chaperones in protein folding and proteostasis. Nature, 475(7356), 324–332. doi:10.1038/nature10317

Recommended Readings: Lei Stanley Qi, Ph.D. Monday, January 13

SPECIAL SEMINAR
Monday, January 13, 2014
4:00 p.m., Carson Family Auditorium

Lei Stanley Qi, Ph.D.
UCSF Systems Biology Fellow
Department of Cellular and Molecular Pharmacology and
UCSF Center for Systems and Synthetic Biology
University of California, San Francisco

Repurposing CRISPR/Cas as a versatile platform for genome engineering and imaging

Recommended Readings:

Empirical Articles

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., … Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science, 339(6121), 819–823. doi:10.1126/science.1231143

Gilbert, L. a, Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., … Qi, L. S. (2013). CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell, 154(2), 442–451. doi:10.1016/j.cell.2013.06.044

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337(6096), 816–21. doi:10.1126/science.1225829

Qi, L., Haurwitz, R. E., Shao, W., Doudna, J. A., & Arkin, A. P. (2012). RNA processing enables predictable programming of gene expression. Nature Biotechnology, 30(10), 1002–6. doi:10.1038/nbt.2355

Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013). Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152(5), 1173–83. doi:10.1016/j.cell.2013.02.022

Review Articles

Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013). ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in Biotechnology, 31(7), 397–405. doi:10.1016/j.tibtech.2013.04.004

Makarova, K. S., Haft, D. H., Barrangou, R., Brouns, S. J. J., Charpentier, E., Horvath, P., … Koonin, E. V. (2011). Evolution and classification of the CRISPR-Cas systems. Nature Reviews Microbiology, 9(6), 467–477. doi:10.1038/nrmicro2577

Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012). RNA-guided genetic silencing systems in bacteria and archaea. Nature, 482(7385), 331–338. doi:10.1038/nature10886

Recommended Readings: Scott Lowe, Ph.D.

Friday Lecture Series

Cancer Initiation and Maintenance Genes

Scott Lowe, Ph.D., chair, Geoffrey Beene Cancer Research Center,

Memorial Sloan Kettering Cancer Center;

investigator, Howard Hughes Medical Institute

January 3, 2014

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Readings

Chicas, A., Kapoor, A., Wang, X., Aksoy, O., Evertts, A. G., Zhang, M. Q., . . . Lowe, S. W. (2012). H3K4 demethylation by Jarid1a and Jarid1b contributes to retinoblastoma-mediated gene silencing during cellular senescence. Proceedings of the National Academy of Sciences of the United States of America, 109(23), 8971-8976

Dow, L. E., & Lowe, S. W. (2012). Life in the fast lane: Mammalian disease models in the genomics era. Cell, 148(6), 1099-1109

Premsrirut, P. K., Dow, L. E., Kim, S. Y., Camiolo, M., Malone, C. D., Miething, C., . . . Lowe, S. W. (2011). A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell, 145(1), 145-158

Sawey, E., Chanrion, M., Cai, C., Wu, G., Zhang, J., Zender, L., . . . Powers, S. (2011). Identification of a therapeutic strategy targeting amplified FGF19 in liver cancer by oncogenomic screening. Cancer Cell, 19(3), 347-358

Xue, W., Kitzing, T., Roessler, S., Zuber, J., Krasnitz, A., Schultz, N., . . . Lowe, S. W. (2012). A cluster of cooperating tumor-suppressor gene candidates in chromosomal deletions. Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8212-8217

Zuber, J., Rappaport, A. R., Luo, W., Wang, E., Chen, C., Vaseva, A. V., . . . Lowe, S. W. (2011). An integrated approach to dissecting oncogene addiction implicates a myb-coordinated self-renewal program as essential for leukemia maintenance. Genes and Development, 25(15), 1628-1640