Recommended Readings: Sean Brady, Ph.D., May 16

Monday Lecture Series
Monday, May 16, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Sean Brady, Ph.D.
Tri-Institutional Associate Professor,
Evnin Associate Professor and Head,
Laboratory of Genetically Encoded Small Molecules,
The Rockefeller University
Early Career Scientist, Howard Hughes Medical Institute

Bioactive Metabolites from the Human Microbiome: Natural Cures or Snake Oil

Recommended Readings:

Cohen, L. J., Kang, H. S., Chu, J., Huang, Y. H., Gordon, E. A., Reddy, B. V. B., … & Brady, S. F. (2015). Functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist. Proceedings of the National Academy of Sciences, 112(35), E4825-E4834. doi: 10.1073/pnas.1508737112.

Charlop-Powers, Z., Milshteyn, A., & Brady, S. F. (2014). Metagenomic small molecule discovery methods. Current Opinion in Microbiology, 19, 70-75. doi: 10.1016/j.mib.2014.05.021.

Recommended Readings: Mary Jeanne Kreek, M.D., May 2

Monday Lecture Series
Monday, May 2, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Mary Jeanne Kreek, M.D.
Senior Attending Physician,
The Rockefeller University Hospital
Patrick E. and Beatrice M. Haggerty Professor and Head,
Laboratory of the Biology of Addictive Diseases,
The Rockefeller University

50th Anniversary of First Research Paper on Methadone Maintenance Treatment: Update on Molecular, Neurobiological, Behavioral, and Genetic Research

Recommended Readings:

Bond, C., LaForge, K. S., Tian, M., Melia, D., Zhang, S., Borg, L., … & Tischfield, J. A. (1998). Single-nucleotide polymorphism in the human mu opioid receptor gene alters β-endorphin binding and activity: possible implications for opiate addiction. Proceedings of the National Academy of Sciences, 95(16), 9608-9613.

Butelman, E. R., Yuferov, V., & Kreek, M. J. (2012). κ-opioid receptor/dynorphin system: genetic and pharmacotherapeutic implications for addiction. Trends in Neurosciences, 35(10), 587-596. doi:10.1016/j.tins.2012.05.005.

Dole, V. P., Nyswander, M. E., & Kreek, M. J. (1966). Narcotic blockade. Archives of Internal Medicine, 118(4), 304-309. doi:10.1001/archinte.1966.00290160004002

Kreek, M. J., Nielsen, D. A., Butelman, E. R., & LaForge, K. S. (2005). Genetic influences on impulsivity, risk taking, stress responsivity and vulnerability to drug abuse and addiction. Nature Neuroscience, 8(11), 1450-1457. doi:10.1038/nn1583

Reed, B., Butelman, E. R., Yuferov, V., Randesi, M., & Kreek, M. J. (2014). Genetics of opiate addiction. Current Psychiatry Reports, 16(11), 1-12. doi:10.1007/s11920-014-0504-6.

Recommended Readings: Tarun Kapoor, Ph.D., April 18

Monday Lecture Series
Monday, April 18, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Tarun Kapoor, Ph.D.
Pels Family Professor and Head,
Selma and Lawrence Ruben Laboratory of Chemistry and Cell Biology,
The Rockefeller University

New Chemical Probes for Ribosome Biogenesis and Cytoskeleton Organization

Recommended Readings

Kasap, C., Elemento, O., & Kapoor, T. M. (2014). DrugTargetSeqR: a genomics-and CRISPR-Cas9–based method to analyze drug targets. Nature Chemical Biology, 10(8), 626-628. doi: 10.1038/nchembio.1551

See, S. K., Hoogendoorn, S., Chung, A. H., Ye, F., Steinman, J. B., Sakata-Kato, T., … & Chen, J. K. (2015). Cytoplasmic dynein antagonists with improved potency and isoform selectivity. ACS Chemical Biology, 11(1), 53-60. doi:10.1021/acschembio.5b00895

Shimamoto, Y., Forth, S., & Kapoor, T. M. (2015). Measuring pushing and braking forces generated by ensembles of kinesin-5 crosslinking two microtubules. Developmental Cell, 34(6), 669-681. doi:10.1016/j.devcel.2015.08.017

Recommended Readings: Rod MacKinnon, M.D., Ph.D., April 11

Monday Lecture Series
Monday, April 11, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Rod MacKinnon, M.D., Ph.D.
John D. Rockefeller Jr. Professor and Head,
Laboratory of Molecular Neurobiology and Biophysics,
The Rockefeller University
Investigator, Howard Hughes Medical Institute

Some Experiments That Excite Me Right Now

Recommended Readings

Brohawn, S. G., Campbell, E. B., & MacKinnon, R. (2014). Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel. Nature, 516(7529), 126-130. doi:10.1038/nature14013

Doyle, D. A., Cabral, J. M., Pfuetzner, R. A., Kuo, A., Gulbis, J. M., Cohen, S. L., … & MacKinnon, R. (1998). The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science, 280(5360), 69-77. doi:10.1126/science.280.5360.69

Hite, R. K., Yuan, P., Li, Z., Hsuing, Y., Walz, T., & MacKinnon, R. (2015). Cryo-electron microscopy structure of the Slo2. 2 Na+-activated K+ channel. Nature, 527(7577), 198-203.  doi:10.1038/nature14958

MacKinnon, R. (2004). Potassium channels and the atomic basis of selective ion conduction (Nobel Lecture). Angewandte Chemie, 43(33), 4265-4277.

Recommended Readings: Paul Cohen, M.D., Ph.D., April 25

Monday Lecture Series
Monday, April 25, 2016
4:00 p.m., Carson Family Auditorium (CRC)

Paul Cohen, M.D., Ph.D.
Albert Resnick, M.D., Assistant Professor and Head,
Laboratory of Molecular Metabolism,
The Rockefeller University

Molecular Regulation of Fat Cell Phenotype

Recommended Readings

Chi, J., & Cohen, P. (2016). The multifaceted roles of PRDM16: Adipose biology and beyond. Trends in Endocrinology & Metabolism, 27(1), 11-23. doi:10.1016/j.tem.2015.11.005

Cohen, P., Levy, J. D., Zhang, Y., Frontini, A., Kolodin, D. P., Svensson, K. J., … & Wu, J. (2014). Ablation of PRDM16 and beige adipose causes metabolic dysfunction and a subcutaneous to visceral fat switch. Cell, 156(1), 304-316. doi:10.1016/j.cell.2013.12.021

Cohen, P., & Spiegelman, B. M. (2015). Brown and beige fat: molecular parts of a thermogenic machine. Diabetes, 64(7), 2346-2351. doi: 10.2337/db15-0318

Kir, S., White, J. P., Kleiner, S., Kazak, L., Cohen, P., Baracos, V. E., & Spiegelman, B. M. (2014). Tumour-derived PTH-related protein triggers adipose tissue browning and cancer cachexia. Nature, 513(7516), 100-104. doi:10.1038/nature13528

Recommended Readings: Paul Bieniasz, Ph.D., December 14th

Monday Lecture Series
Monday, December 14, 2015
4:00 p.m., Carson Family Auditorium (CRC)

Paul Bieniasz, Ph.D.
Professor and Head,
Laboratory of Retrovirology,
The Rockefeller University and Aaron Diamond AIDS Research Center
Investigator, Howard Hughes Medical Institute

Retroviruses vs. Cells in Ancient and Modern Times

Recommended Reading

Empirical Articles

Busnadiego, I., Kane, M., Rihn, S. J., Preugschas, H. F., Hughes, J., Blanco-Melo, D., … & Wilson, S. J. (2014). Host and viral determinants of Mx2 antiretroviral activity. Journal of Virology, 88(14), 7738-7752. doi:10.1128/JVI.00214-14.

Hatziioannou, T., Del Prete, G. Q., Keele, B. F., Estes, J. D., McNatt, M. W., Bitzegeio, J., … & Bieniasz, P. D. (2014). HIV-1–induced AIDS in monkeys. Science, 344(6190), 1401-1405. doi:10.1126/science.1250761.

Kane, M., Yadav, S. S., Bitzegeio, J., Kutluay, S. B., Zang, T., Wilson, S. J., … & Bieniasz, P. D. (2013). MX2 is an interferon-induced inhibitor of HIV-1 infection. Nature, 502(7472), 563-566. doi:10.1038/nature12653.

Kutluay, S. B., Zang, T., Blanco-Melo, D., Powell, C., Jannain, D., Errando, M., & Bieniasz, P. D. (2014). Global changes in the RNA binding specificity of HIV-1 gag regulate virion genesis. Cell, 159(5), 1096-1109. doi:10.1016/j.cell.2014.09.057.

Rihn, S. J., Hughes, J., Wilson, S. J., & Bieniasz, P. D. (2015). Uneven genetic robustness of HIV-1 integrase. Journal of Virology, 89(1), 552-567. doi: 10.1128/JVI.02451-14.

Review Papers

Malim, M. H., & Bieniasz, P. D. (2012). HIV restriction factors and mechanisms of evasion. Cold Spring Harbor Perspectives in Medicine, 2(5), a006940.  doi:10.1101/cshperspect.a006940.

Recommended Readings: Titia de Lange, Ph.D., December 7th

Monday Lecture Series
Monday, December 7, 2015
4:00 p.m., Carson Family Auditorium (CRC)

Titia de Lange, Ph.D.
Leon Hess Professor and Head,
Laboratory of Cell Biology and Genetics,
The Rockefeller University

Telomere-derived Genome Instability in Cancer

Recommended Readings

Davoli, T., & de Lange, T. (2011). The causes and consequences of polyploidy in normal development and cancer. Annual review of cell and developmental biology, 27, 585-610. doi:10.1146/annurev-cellbio-092910-154234

Davoli, T., & de Lange, T. (2012). Telomere-driven tetraploidization occurs in human cells undergoing crisis and promotes transformation of mouse cellsCancer Cell, 21(6), 765-776. doi:10.1016/j.ccr.2012.03.044

de Lange, T. (2005). Telomere-related genome instability in cancer. Cold Spring Harbor Symposia on Quantitative Biology, 70, 197-204. doi:10.1101/sqb.2005.70.032

Recommended Readings: David C. Gadsby, Ph.D., November 30th

Monday Lecture Series
Monday, November 30, 2015
4:00 p.m., Carson Family Auditorium (CRC)

David C. Gadsby, Ph.D.
Patrick A. Gerschel Family Professor and Head
Laboratory of Cardiac and Membrane Physiology,
The Rockefeller University

Motions that gate single CFTR channels, the ABC proteins whose failure causes cystic fibrosis

Recommended Readings

Chaves, L. A. P., & Gadsby, D. C. (2015). Cysteine accessibility probes timing and extent of NBD separation along the dimer interface in gating CFTR channels. The Journal of General Physiology, 145(4), 261-283. doi:10.1085/jgp.201411347

Csanády, L., Vergani, P., & Gadsby, D. C. (2010). Strict coupling between CFTR’s catalytic cycle and gating of its Cl− ion pore revealed by distributions of open channel burst durations. Proceedings of the National Academy of Sciences, 107(3), 1241-1246. doi:10.1073/pnas.0911061107

Gadsby, D. C., Vergani, P., & Csanády, L. (2006). The ABC protein turned chloride channel whose failure causes cystic fibrosis. Nature, 440(7083), 477-483. doi:10.1038/nature04712

Vergani, P., Lockless, S. W., Nairn, A. C., & Gadsby, D. C. (2005). CFTR channel opening by ATP-driven tight dimerization of its nucleotide-binding domains. Nature, 433(7028), 876-880. doi:10.1038/nature03313

Recommended Readings: Robert G. Roeder, Ph.D., November 16th

Monday Lecture Series
Monday, November 16, 2015
4:00 p.m., Carson Family Auditorium (CRC)

Robert G. Roeder, Ph.D.
Arnold and Mabel Beckman Professor and Head,
Laboratory of Biochemistry and Molecular Biology,
The Rockefeller University

Transcriptional Regulatory Mechanisms in Animal Cells

Recommended Readings

Cevher, M. A., Shi, Y., Li, D., Chait, B. T., Malik, S., & Roeder, R. G. (2014). Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit. Nature Structural & Molecular Biology, 21(12), 1028-34. doi: 10.1038/nsmb.2914

Deshpande, A. J., Deshpande, A., Sinha, A. U., Chen, L., Chang, J., Cihan, A., … & Armstrong, S. A. (2014). AF10 regulates progressive H3K79 methylation and HOX gene expression in diverse AML subtypes. Cancer Cell, 26(6), 896-908. doi: 10.1016/j.ccell.2014.10.009

Iida, S., Chen, W., Nakadai, T., Ohkuma, Y., & Roeder, R. G. (2015). PRDM16 enhances nuclear receptor-dependent transcription of the brown fat-specific Ucp1 gene through interactions with Mediator subunit MED1. Genes & Development, 29(3), 308-321. doi:10.1101/gad.252809.114

Sabari, B. R., Tang, Z., Huang, H., Yong-Gonzalez, V., Molina, H., Kong, H. E., … & Allis, C. D. (2015). Intracellular Crotonyl-CoA Stimulates Transcription through p300-Catalyzed Histone Crotonylation. Molecular Cell, 58(2), 203-215. doi:10.1016/j.molcel.2015.02.029

Zhang, P., Tu, B., Wang, H., Cao, Z., Tang, M., Zhang, C., … & Zhu, W. G. (2014). Tumor suppressor p53 cooperates with SIRT6 to regulate gluconeogenesis by promoting FoxO1 nuclear exclusion. Proceedings of the National Academy of Sciences, 111(29), 10684-10689. doi:10.1073/pnas.1411026111

Recommended Readings: Jeffrey V. Ravetch, M.D., Ph.D., November 9th

Monday Lecture Series
Monday, November 9, 2015
4:00 p.m., Carson Family Auditorium (CRC)

Jeffrey V. Ravetch, M.D., Ph.D.
Theresa and Eugene M. Lang Professor and Head,
Laboratory of Molecular Genetics and Immunology,
The Rockefeller University

Diversification of antibody effector function

Recommended Readings

Empirical Articles

Bournazos, S., Klein, F., Pietzsch, J., Seaman, M. S., Nussenzweig, M. C., & Ravetch, J. V. (2014). Broadly neutralizing anti-HIV-1 antibodies require Fc effector functions for in vivo activity. Cell, 158(6), 1243-1253. doi: 10.1016/j.cell.2014.08.023

DiLillo, D. J., & Ravetch, J. V. (2015). Differential Fc-receptor engagement drives an anti-tumor vaccinal effect. Cell, 161(5), 1035-1045. doi:10.1016/j.cell.2015.04.016

Fiebiger, B. M., Maamary, J., Pincetic, A., & Ravetch, J. V. (2015). Protection in antibody-and T cell-mediated autoimmune diseases by antiinflammatory IgG Fcs requires type II FcRs. Proceedings of the National Academy of Sciences, 112(18), E2385-E2394. doi:10.1073/pnas.1505292112

Wang, T. T., Maamary, J., Tan, G. S., Bournazos, S., Davis, C. W., Krammer, F., … & Ravetch, J. V. (2015). Anti-HA glycoforms drive B cell affinity selection and determine influenza vaccine efficacy. Cell, 162(1), 160-169. doi:10.1016/j.cell.2015.06.026

Review Papers

Bournazos, S., DiLillo, D. J., & Ravetch, J. V. (2015). The role of Fc–FcγR interactions in IgG-mediated microbial neutralization. The Journal of Experimental Medicine, 212(9), 1361-1369. doi:10.1084/jem.20151267

Pincetic, A., Bournazos, S., DiLillo, D. J., Maamary, J., Wang, T. T., Dahan, R., … & Ravetch, J. V. (2014). Type I and type II Fc receptors regulate innate and adaptive immunity. Nature Immunology, 15(8), 707-716. doi:10.1038/ni.2939